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1290 uhplc system

Manufactured by AB Sciex
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The 1290 UHPLC system is a high-performance liquid chromatography instrument designed by AB Sciex. It features rapid separation and analysis capabilities, enabling efficient and precise quantification of analytes in complex samples.

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Lab products found in correlation

4000 qtrap mass spectrometer, by AB Sciex (2 mentions)

2 protocols using 1290 uhplc system

1

Metabolite Profiling by UPLC-MS/MS

Cited 1 time
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Metabolite measurements were performed by ultrahigh-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) on an Agilent 1290 UHPLC system coupled to a Sciex 4000 QTRAP mass spectrometer. For sample preparation, 45 μl of each sample was mixed with 105 μl of acetonitrile. After vortex mixing for 15 s and sonication in an ice-water bath for 1 min, the samples were centrifuged (14,000 x g, 10 min, 4 °C). The supernatants were used for quantitation of the metabolites using two different UPLC-MRM/MS methods, as described13 (link) (Supplementary file 1, Supplementary methods).
Boczonadi V., King M.S., Smith A.C., Olahova M., Bansagi B., Roos A., Eyassu F., Borchers C., Ramesh V., Lochmüller H., Polvikoski T., Whittaker R.G., Pyle A., Griffin H., Taylor R.W., Chinnery P.F., Robinson A.J., Kunji E.R, & Horvath R. (2018). Mitochondrial oxodicarboxylate carrier deficiency is associated with mitochondrial DNA depletion and spinal muscular atrophy-like disease. Genetics in medicine : official journal of the American College of Medical Genetics, 20(10), 1224-1235.
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2

Metabolite Profiling by UPLC-MS/MS

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Metabolite measurements were performed by ultrahigh-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) on an Agilent 1290 UHPLC system coupled to a Sciex 4000 QTRAP mass spectrometer. For sample preparation, 45 μl of each sample was mixed with 105 μl of acetonitrile. After vortex mixing for 15 s and sonication in an ice-water bath for 1 min, the samples were centrifuged (14,000 x g, 10 min, 4 °C). The supernatants were used for quantitation of the metabolites using two different UPLC-MRM/MS methods, as described13 (link) (Supplementary file 1, Supplementary methods).
Boczonadi V., King M.S., Smith A.C., Olahova M., Bansagi B., Roos A., Eyassu F., Borchers C., Ramesh V., Lochmüller H., Polvikoski T., Whittaker R.G., Pyle A., Griffin H., Taylor R.W., Chinnery P.F., Robinson A.J., Kunji E.R, & Horvath R. (2018). Mitochondrial oxodicarboxylate carrier deficiency is associated with mitochondrial DNA depletion and spinal muscular atrophy-like disease. Genetics in medicine : official journal of the American College of Medical Genetics, 20(10), 1224-1235.
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