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1 ethyl 3 dimethylaminopropyl carbodiimide hydrochloride

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1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride is a water-soluble carbodiimide compound commonly used as a coupling agent in various biochemical and organic synthesis applications. It facilitates the formation of amide bonds between carboxyl and amino groups.

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Lab products found in correlation

N hydroxysuccinimide, by Merck Group (3 mentions) Triethylamine, by Merck Group (2 mentions) Ofloxacin, by Merck Group (1 mentions) Gatifloxacin, by Merck Group (1 mentions) Norfloxacin, by Merck Group (1 mentions) Danofloxacin, by Merck Group (1 mentions) Pefloxacin, by Merck Group (1 mentions) Sarafloxacin, by Merck Group (1 mentions) Lomefloxacin, by Merck Group (1 mentions) Tosufloxacin, by Merck Group (1 mentions) Sparfloxacin, by Merck Group (1 mentions) Difloxacin, by Merck Group (1 mentions) Marbofloxacin, by Merck Group (1 mentions) Pipemidic acid, by Merck Group (1 mentions) Oxolinic acid, by Merck Group (1 mentions) Orbifloxacin, by Merck Group (1 mentions) Enoxacin, by Merck Group (1 mentions) Nadifloxacin, by Merck Group (1 mentions) Flumequine, by Merck Group (1 mentions) Glutardialdehyde, by Merck Group (1 mentions)

4 protocols using 1 ethyl 3 dimethylaminopropyl carbodiimide hydrochloride

1

Development of Fluoroquinolone Immunoassays

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All of the chemicals used in this investigation were of analytical grade. Danofloxacin (DAN), ofloxacin (OFL), levofloxacin (LEV), garenoxacin (GAR), pefloxacin (PEF), gatifloxacin (GAT), clinafloxacin (CLI), sarafloxacin (SAR), lomefloxacin (LOM), tosufloxacin (TOZ), sparfloxacin (SPA), difloxacin (DIF), pazufloxacin (PAZ), marbofloxacin (MAR), moxifloxacin (MOX), rufloxacin (RUF), norfloxacin (NOR), ciprofloxacin (CIP), enrofloxacin (ENR), pipemidic acid (PIP), nalidixic acid (NAL), oxolinic acid (OXO), orbifloxacin (ORB), enoxacin (ENO), nadifloxacin (NAD), flumequine (FLU), bovine serum albumin (BSA), ovalbumine (OVA), casein, 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), N,N-dimethylformamide (DMF), ethylenediamine hydrochloride, triethylamine, sodium borohydride, glutardialdehyde, 4-aminomethylfluorescein (4-AMF), 3,3′,5,5′-tetramethylbenzidine (TMB), and Tween-20 were Sigma-Aldrich (St. Louis, MO, USA) products. Complete and incomplete Freund’s adjuvants were produced by Becton Dickinson (Franklin Lakes, NJ, USA). Peroxidase-labeled anti-rabbit immunoglobulins were from the Gamaleya Institute of Microbiology and Epidemiology (Moscow, Russia). All other chemicals (salts and solvents of analytical grade) were from Khimmed (Moscow, Russia).
Buglak A.A., Shanin I.A., Eremin S.A., Lei H.T., Li X., Zherdev A.V, & Dzantiev B.B. (2019). Ciprofloxacin and Clinafloxacin Antibodies for an Immunoassay of Quinolones: Quantitative Structure–Activity Analysis of Cross-Reactivities. International Journal of Molecular Sciences, 20(2), 265.
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2

Hyaluronic Acid-Based Anticancer Drug Delivery

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HA (molecular weight [Mw] =100 kDa) was obtained from Sunlidabio Ltd (Nanjing, China). Mouse breast tumor cell line (4T1) was obtained from the Chinese Academy of Science Cell Bank for Type Culture Collection (Shanghai, China). FA, doxorubicin hydrochloride (DOX·HCl), ethylene diamine, glycidyl methacrylate (GM), reduced l-glutathione (GSH), 2,2-dimethoxy-2-phenylacetophenone, cystamine dihydrochloride, triethylamine, 1-hydroxybenzotriazole, (1-ethyl-3-[dimethylamino]propyl)carbodiimide hydrochloride, N,N′-dicyclohexylcarbodiimide, N-hydroxysuccinimide, and other chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA).
Yi Q., Ma J., Kang K, & Gu Z. (2018). Bioreducible nanocapsules for folic acid-assisted targeting and effective tumor-specific chemotherapy. International Journal of Nanomedicine, 13, 653-667.
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3

Chitosan-based Bioactive Compounds Synthesis

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Low molecular weight chitosan (LCS) is a commercial material provided by Qingdao Yunzhou Biotechnology Co., Ltd. (China). It's deacetylation degree >90%, average molecular weight (Mw) of 3 kDa. Chitooligosaccharide (COS) with a molecular weight of 1.1 kDa was prepared in our laboratory by the combined degradation of microwave and acetic acid. Tris (Tris), Reduced Coenzyme I (NADH), Safranine O, Nitrotetrazolium Blue (NBT), Phenazine Potassium Sulfate (PMS), 1,1-Diphenyl-2-Trinitrophenylhydrazine (DPPH) was purchased from Sigma Chemicals Co. Disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, ferrous sulfate, disodium ethylenediaminetetraacetate (EDTA), absolute ethanol, 30% hydrogen peroxide (H2O2), 1-ethyl-(3 -Dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES), salicylic acid (BHA), α-naphthylacetic acid (NAA), indole-3-butyric acid (IBA). All other chemicals and reagents were of analytical grade and were used without further purification.
Liu Y., Wen F., Yang H., Bao L., Zhao Z, & Zhong Z. (2022). The preparation and antioxidant activities of three phenyl-acylchitooligosaccharides. Heliyon, 8(9), e10624.
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4

Biomolecular Conjugation for TKI Analysis

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Human serum albumin (HSA), 16-Mercaptohexadecanoic acid (MHA), 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC), and N-hydroxysulfosuccinimide (NHS) were all obtained from Sigma Aldrich. TKIs (imatinib, nilotinib, dasatinib, bosutinib, and ponatinib) were obtained from MedChemExpress (MCE). Gold-coated silicon wafers were obtained from TED PELLA, INC, and AFM probes were supplied by Budget Sensors (Sofia, Bulgaria). Phosphate-buffered saline (10 mM PBS, 140 mM NaCl, 3 mM KCI, pH 7.4) and ethanol (guaranteed grade) were supplied by Merck Co. (Readington Township, NJ, USA), and Millipore water and analytical grade reagents were used throughout the study.
Fu Y., Wang J., Wang Y, & Sun H. (2022). Investigating the Effect of Tyrosine Kinase Inhibitors on the Interaction between Human Serum Albumin by Atomic Force Microscopy. Biomolecules, 12(6), 819.
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