Transfections of GFP-RNase H1 plasmid into human HeLa and mouse embryonic fibroblasts (MEF) cells were carried out as described previously4 . Ago2 KO and parental wild type cells are MEFs. G9a/GLP double KO and their parental wild type are mouse embryonic stem (mES) cells. Treatment with 10 μM of BIX-01294 inhibitor (Sigma) was performed as described20 (link). Total RNA was isolated using TRIzol reagent (Invitrogen) and reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) using gene-specific primers. J2 dsRNA pull-down was performed as described8 (link). RT-qPCR levels are presented graphically as raw values x1000. Chromatin immuno-precipitation (ChIP) and genomic DNA immuno-precipitation (DIP) analyses were carried out as before4 . The following antibodies were used for ChIP: anti-H3K9me2 (Abcam), anti-H3K9me3 (Abcam), anti-H3 (Abcam), anti-Dicer (13D6) (Abcam), anti-KMT1C/G9a (Abcam), anti-Ago1 (Millipore), anti-Ago2 (Abcam) and anti-Pol II (H-224) (Santa Cruz Biotechnology). S9.6 RNA:DNA hybrid specific antibody was used for DIP4
Anti kmt1c g9a
Manufactured by Abcam
Anti-KMT1C/G9a is a primary antibody that specifically binds to the KMT1C/G9a protein, which is a histone methyltransferase enzyme responsible for mono- and di-methylation of histone H3 at lysine 9 (H3K9me1 and H3K9me2).
Automatically generated - may contain errors
Lab products found in correlation
Superscript 3 reverse transcriptase, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Anti ago1, by Merck Group (2 mentions)
Anti ago2, by Abcam (2 mentions)
Bix 01294 inhibitor, by Merck Group (2 mentions)
Anti pol 2 h 224, by Santa Cruz Biotechnology (2 mentions)
Anti h3k9me2, by Abcam (2 mentions)
Anti dicer 13d6, by Abcam (2 mentions)
Anti h3k9me3, by Abcam (2 mentions)
Anti h3, by Abcam (2 mentions)
2 protocols using anti kmt1c g9a
1
Epigenetic Analysis of GFP-RNase H1 in Mammalian Cells
2
Epigenetic Analysis of GFP-RNase H1 in Mammalian Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Transfections of GFP-RNase H1 plasmid into human HeLa and mouse embryonic fibroblasts (MEF) cells were carried out as described previously4 . Ago2 KO and parental wild type cells are MEFs. G9a/GLP double KO and their parental wild type are mouse embryonic stem (mES) cells. Treatment with 10 μM of BIX-01294 inhibitor (Sigma) was performed as described20 (link). Total RNA was isolated using TRIzol reagent (Invitrogen) and reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) using gene-specific primers. J2 dsRNA pull-down was performed as described8 (link). RT-qPCR levels are presented graphically as raw values x1000. Chromatin immuno-precipitation (ChIP) and genomic DNA immuno-precipitation (DIP) analyses were carried out as before4 . The following antibodies were used for ChIP: anti-H3K9me2 (Abcam), anti-H3K9me3 (Abcam), anti-H3 (Abcam), anti-Dicer (13D6) (Abcam), anti-KMT1C/G9a (Abcam), anti-Ago1 (Millipore), anti-Ago2 (Abcam) and anti-Pol II (H-224) (Santa Cruz Biotechnology). S9.6 RNA:DNA hybrid specific antibody was used for DIP4
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