Lumikine xpress mifn β 2.0 kit

For qPCR, total RNA was extracted using QIAshredder and the RNeasy Plus Mini Kit (catalog 79654 and 74134, respectively; Qiagen). RNA concentration was measured using a Nanodrop Spectrophotometer (Thermo Fisher Scientific). RNA was reverse-transcribed into cDNA using High-Capacity cDNA Reverse Transcription Kit and RNAse inhibitor (4368814 and N8080119, Applied Biosystems). The primers were synthesized by Integrated DNA Technologies. The primer sequences are shown in Table S2. For ELISA, supernatant from treated MOC2-E6/E7, BMDMs, and peritoneal macrophages was collected 16 h post-treatment with agonists of the type I interferon pathway. Protein levels of murine Ifn-β and Tnf-α in the supernatant were quantified using a LumiKine^™ Xpress mIFN-β 2.0 kit (luex-mifnbv2, Invivogen) and Tnf-α Mouse ELISA kit (BMS6073, Invitrogen). The bioluminescent intensity was measured using a Synergy H1 microplate reader (Agilent) and Gen5 program (version 2.09), and three technical replicates per sample were examined simultaneously.