WT C57BL/6J mice were obtained by Charles River Laboratories (Calco, Italy). Mice with macrophage-specific Ms4a4a inactivation in C57BL/6J background were achieved breading mice carrying floxed Ms4a4a alleles (Ms4a4afl/fl; Ozgene) with mice expressing Cre under the control of the promoter of the lys2 gene, encoding for the myeloid-restricted lysozyme M protein (LysCre/+). Ms4a4afl/flLysCre/+ mice (here indicated as Ms4a4a-/-) were born in mendelian ratios, reproduced normally, and did not show significant differences in body weight compared to control floxed littermates (Ms4a4afl/flLys+/+, here used as WT animals). Ms4a4a-/- mice were co-housed with littermates in individually ventilated cages in a specific pathogen-free/viral antibody-free animal facility at Humanitas Clinical and Research Center. Clec7a-/- mice (here indicated as Dectin-1-/-) were obtained by Jackson Laboratory. To obtain double knock-out mice (here indicated as Ms4a4a x Dectin-1 KO), Dectin-1-/- mice were bred with Ms4a4afl/fl x ubiquitinCre/+ mice. Animals were housed in ventilated cages in a specific pathogen-free/viral antibody-free animal facility at Humanitas Clinical and Research Center. Experiments were performed using sex- and age-matched mice.
C57bl 6j wt mice
Manufactured by Charles River Laboratories
Sourced in Germany, China, France, Japan, United States, United Kingdom
C57BL/6J WT mice are an inbred strain of laboratory mice commonly used in biomedical research. They serve as a standard reference strain for genetic and phenotypic comparisons.
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Lab products found in correlation
C57bl 6j, by Jackson ImmunoResearch (3 mentions)
Clec7a mice, by Jackson ImmunoResearch (1 mentions)
Wt cd 1 mice, by Charles River Laboratories (1 mentions)
C57bl 6 ackr3tm1litt j, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
B6 cg tg thy1 cop4 eyfp 18gfng j thy1 chr2, by Jackson ImmunoResearch (1 mentions)
Rosa tdtomato mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6j mice, by GemPharmatech (1 mentions)
Apcmin mice, by Jackson ImmunoResearch (1 mentions)
Villin cre, by Jackson ImmunoResearch (1 mentions)
Lgr5 egfp ires creert2, by Jackson ImmunoResearch (1 mentions)
Cd 1 icr, by Charles River Laboratories (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Rag2 mice, by Taconic Biosciences (1 mentions)
K14 cretg 0 mice, by Jackson ImmunoResearch (1 mentions)
Il4ra mice, by Jackson ImmunoResearch (1 mentions)
Mcpt8cre mice, by Jackson ImmunoResearch (1 mentions)
Il1r1, by Jackson ImmunoResearch (1 mentions)
B6.129s2 trp53tm1tyj j, by Jackson ImmunoResearch (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
61 protocols using c57bl 6j wt mice
1
Macrophage-specific Ms4a4a Knockout Mice
2
Mouse Models for Cancer Research
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WT C57BL/6J mice were purchased from Charles River Laboratories Japan and CLEA Japan. SCID-Beige (CB17.Cg-PrkdcscidLystbg-J/CrlCrlj) mice were purchased from Charles River Laboratories Japan. p16/p21-DKO mice were generated as previously reported9 (link). p16-luc mice11 (link) and p21-luc mice10 (link) were generated as previously reported and backcrossed with C57BL/6 mice for at least 8 generations. All animals were maintained according to protocols approved by the Committee for the Use and Care of Experimental Animals of the Japanese Foundation for Cancer Research (Tokyo, Japan) and the Research Institute for Microbial Diseases, Osaka University (Osaka, Japan).
3
Standardized Mouse Embryo Experiments
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Experiments, with the exception of the HCR experiment (see below), were performed in accordance with EU guidelines for the care and use of laboratory animals, and under the authority of appropriate UK governmental legislation. Eight- to 12-week-old WT C57BL/6J mice (Charles Rivers) were used, with the exception of the HCR experiment. For the HCR experiment, WT CD-1 mice (Charles Rivers) were used. Natural mating was set up between males and 4- to 6-week-old virgin females, with 12:00 of the day of vaginal plug considered to be E0.5. Mice were maintained in accordance with guidelines from Memorial Sloan Kettering Cancer Center (MSKCC) Institutional Animal Care and Use Committee (IACUC) under protocol number 03-12-017 (principal investigator A.-K.H.). All mice used in this project were housed under a 12-h light/12-h dark cycle, with constant access to food and water. No sex selection of the used embryos was performed.
4
Mouse Models for Influenza Research
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Female and male WT C57BL/6J mice and Il1rap−/− mice on the C57BL/6 background or Il36r−/− mice or WT Balb/cAnCrl mice, all 8–13 weeks of age, were purchased from Charles River (Sulzfeld, Germany or the US). Animals were housed in groups of 5 mice per cage under specific pathogen-free conditions in isolated ventilated cages at 20–25 °C and a humidity of 46–65% with a dark/night cycle of 12 h. Mice had free access to water and chow. All experiments were approved by the animal welfare officers within Boehringer Ingelheim Pharma GmbH and Co KG, as well as by the local authorities for the care and use of experimental animals (Regierungspräsidium Tübingen; TVV 12-009-G and 14-016-G; 35/9185.81-8). Experiments with influenza virus were performed under biosafety level 2 conditions and were in accordance with German national guidelines and legal regulations.
5
Transgenic Mouse Model for CD5 Studies
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In vivo studies were carried out at the animal facilities of the School of Medicine of the University of Barcelona under protocols approved by the Ethics Committee for Animal Research of the University of Barcelona (permits number 740/14, 741/14 and 54/16). Homozygous shCD5EμTg transgenic mice of C57BL/6 genetic background were obtained by intercrossing of previously reported heterozygous mice [24 (link)]. The homozygosity of shCD5EμTg mice was indirectly ascertained from their offspring breeding homozygous candidates with non-transgenic mice (NonTg). NonTg mice used for comparative purposes came from the same common heterozygous ancestors as the shCD5EμTg mice and were kept under the same housing conditions as the latter. For some experiments WT C57BL/6J mice were purchased from Charles River. All animals were maintained under conventional (non-specific-pathogen-free) housing conditions.
6
DMBA-Induced Tumor Formation in GABARAP KO Mice
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Wt C57BL/6J mice were purchased from Charles River (Sulzfeld, Germany). GABARAP KO mice were established from a colony initially maintained at the Max Planck Institute for Brain Research (Frankfurt, Germany). The strain background of the GABARAP-deficient mice is the C57BL/6J strain. As previously reported, GABARAP KO mice were phenotypically indistinguishable from Wt mice and showed no detectable changes in gross anatomy.6 (link) DMBA (Sigma-Aldrich, Taufkirchen, Germany) was dissolved in sesame oil (5 mg/ml). Mice at 6–8 weeks of age received 6 weekly doses of 1 mg DMBA/mouse by oral gavage. Vehicle-treated (control) groups received just sesame oil. The mice were monitored weekly for palpable tumor formation. Tumor specimens were fixed and then stained with hematoxylin and eosin. All animal experiments were approved by the local government commission for animal protection (No. 02-018/08 and 02-007/13).
7
Transgenic Mouse Strains for Neurobiology Research
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WT (C57BL/6J) mice were purchased from Charles River Laboratory (219, Beijing, China), Shank3−/− mice (017688), BTBR mice (002282), Agrp-Cre mice (012899), POMC-Cre mice (010714) were purchased from Jax lab (USA), p38αD176A−F327S and p38αT180A−Y182F flox knock-in mice were generated by GemPharmatech (Nanjing, China), p38αflox/flox knock-in mice were kindly provided by Lijian Hui [32 (link)], and Tomato-reporter mice were purchased from GemPharmatech (T002249, Nanjing, China). All animals were kept under standard conditions with temperature (22 ± 1 °C) and humidity (~ 40%) in a 12 h light/12 hours dark cycle, with free access to food and water. Mice were used for experiments at 10–12 weeks of age. All animal experiments were permitted by the Institutional Animal Care and Use Committee at Shandong Provincial Hospital and complied with the China National Regulations on the Administration of Experimental or Laboratory Animals (No.2, 20,170,301, SSTC, China), and the ARRIVE guidelines or the U.K. Animals (Scientific Procedures) Act, 1986.
8
Genetic and Toxin Models of Parkinson's Disease
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Twelve-month-old male and female A53T-OE mice and WT littermates and 22-mo-old male and female VMAT2-LO mice and WT littermates were used for studies involving genetic models of PD. Six- to twelve-month-old male WT C57BL/6J mice (Charles River Laboratories) were used for MPTP studies. Three to six animals were included in each group for all experiments. All procedures were conducted in accordance with the National Institutes of Health Guide for Care and Use of Laboratory Animals (62 ) and were previously approved by the Institutional Animal Care and Use Committee at Emory University.
9
Genetic Mouse Models for Cancer Research
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WT C57BL/6J mice were from Charles River Laboratories. ACKR3-GFP reporter mice were from The Jackson Laboratory (C57BL/6-Ackr3tm1Litt/J) (Cruz-Orengo et al., 2011 (link)). Cyp19Cre mice were a generous gift from Prof. Gustavo Leone (Wenzel and Leone, 2007 (link)) and ‘floxed’ ACKR3 mice, B6.Cg-Ackr3tm1Fma/J, were obtained from The Jackson Laboratory. We used 7-10 week-old female mice for timed mating. Mice were maintained in specific pathogen-free facilities at the Beatson Institute and University of Glasgow. All experiments were performed under a UK Home Office Project License and approved by local ethical review committee at the University of Glasgow.
10
Immune Response to RNA Vaccine in Mice
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MyD88–/– mice (C57Bl6/J background) were provided by S. Akira (Laboratory of Host Defense, Immunology Frontier Research Center, Osaka University). WT C57Bl6/J mice were purchased from Charles River Laboratories. All mice were housed in the specific pathogen–free animal facility of the Department of Medicine, University of Verona. Sex- and age-matched WT and MyD88–/– mice (8–10 weeks old) were anesthetized with isoflurane and i.v. injected in the retro-orbital vein with 300 μL DOTAP/SCV2-RNA mixture (20 μg/mouse) or with DOTAP alone. After 6 hours, mice were euthanized and lungs, spleen, and blood were harvested. Briefly, lungs were collected upon intracardiac perfusion with cold PBS. Left lung lobes were formalin-fixed for 24 hours, dehydrated, and paraffin-embedded for histological analysis. Right lungs were immediately frozen at –80°C and used for real-time PCR. Spleens were mechanically and enzymatically treated to obtain a single-cell suspension for cytofluorimetric and real-time PCR analysis.
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