Demountable cell

MLV were prepared by rehydrating the resulting films with D₂O or Tris-HCl buffer (10 mM pH 7.0) for FT-IR experiments as described previously (Nasir et al., 2016 (link)). Lipids were co-dissolved in chloroform/methanol (2:1, v/v) without or with peptides at a 10-to-1, lipid-to-peptide molar ratio. FTIR spectra of lipid-peptide MLV were recorded on Bruker Equinox 55 spectrometer (Karlsruhe, Germany) equipped with a liquid nitrogen-cooled Deuterated Triglycine Sulfate (DTGS) detector. The spectra were measured with a spectral resolution of 4 cm⁻¹ and are an average of 128 scans. All the experiments were performed with a demountable cell (Bruker) equipped with CaF2 windows (Nasir et al., 2013 (link)). During the experiments, the spectrophotometer was continuously purged with filtered dry air. MLV solution containing or not peptides was deposited into the CaF₂ window-equipped cell. All FTIR spectra were representative of at least two independent measurements. The attribution of different peaks was carried out according to the literature (Arrondo and Goñi, 1998 (link); Kong and Yu, 2007 (link)).

Experiments were carried out on a Bruker Equinox 55 spectrometer (Karlsruhe, Germany) equipped with a liquid-nitrogen-cooled DTGS (deuterated tri glycine sulfate) detector, and continuously purged with N₂ during data acquisition. For each experiment, 128 scans were realized with a 4 cm⁻¹ resolution at room temperature A demountable cell (Bruker) equipped with CaF₂ windows was used for all experiments. At least two independent measurements were realized to assure results accuracy.