Cd49e pe

Manufactured by Thermo Fisher Scientific

CD49e PE is a fluorescent-conjugated monoclonal antibody that binds to the CD49e (α5 integrin subunit) antigen expressed on various cell types. It is commonly used in flow cytometry applications for the identification and characterization of cells expressing the CD49e marker.

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Lab products found in correlation

Facscanto 2, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Ter119 pe, by Thermo Fisher Scientific (1 mentions) Cd49d fitc, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Facsaria, by BD (1 mentions) Cd44 apc, by Thermo Fisher Scientific (1 mentions) Cd105 pe, by Thermo Fisher Scientific (1 mentions) Cd29 bv510, by BD (1 mentions) Cd61 fitc, by Thermo Fisher Scientific (1 mentions) Cd90 pe cy7, by Thermo Fisher Scientific (1 mentions)

2 protocols using cd49e pe

Erythroid Cell Development Analysis

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Bone marrow, spleen and fetal liver cells were stained for erythroid subsets using the antibodies listed below. Samples were analyzed by a FACSCantoII flow cytometer using FACSDiva software (Becton-Dickinson). Erythrocyte development in the bone marrow and spleen was analyzed as follows: Ter119^medCD71^high (proerythroblasts; R1), Ter119^highCD71^high (basophilic erythroblasts; R2), Ter119^highCD71^med (late basophilic and polychromatophilic erythroblasts; R3) and Ter119^highCD71^low (orthochromatophilic erythroblasts; R4). Cells were stained with the fluorescently labeled antibodies: Ter119-PE (eBioscience), CD71 Biotin (eBioscience), Streptavidin eflour 780 (eBioscience), CD49e PE (integrin α5), and CD49d FITC (integrin α4).

Uras I.Z., Scheicher R.M., Kollmann K., Glösmann M., Prchal-Murphy M., Tigan A.S., Fux D.A., Altamura S., Neves J., Muckenthaler M.U., Bennett K.L., Kubicek S., Hinds P.W., von Lindern M, & Sexl V. (2017). Cdk6 contributes to cytoskeletal stability in erythroid cells. Haematologica, 102(6), 995-1005.

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Comprehensive Stem Cell Marker and Integrin Profiling

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Cells were first stained with fixable viability dye e780 (e-bioscience) according to manufacturer's instructions. Cells were then fixed with 4% paraformaldehyde (PFA) for 15 min prior to incubating the cells with the following conjugated antibodies to test for the expression of known stem cell markers: CD90 PE-Cy7 (e-bioscience), CD105 PE (ebioscience), CD44 APC (e-bioscience), CD73 e450 (e-bioscience), TRA-1-60 PerCP e710 (e-bioscience), OCT4 e660 (e-bioscience).
Additionally, the following antibodies were used to characterize the expression of integrin binding proteins: CD49b BV421 (BD Bioscience), CD29 BV510 (BD Bioscience), CD61 FITC (e-bioscience), CD49e PE (ebioscience), CD104 e660 (e-bioscience), CD49a PerCP e710 (e-bioscience) and CD49f PE-Cy7 (e-bioscience). All samples were run and analyzed on a BD LSRII flow cytometer using BD FACSAria.

Jensen T., Wanczyk H., Sharma I., Mitchell A., Sayej W.N, & Finck C. (2019). Polyurethane scaffolds seeded with autologous cells can regenerate long esophageal gaps: An esophageal atresia treatment model. Journal of pediatric surgery, 54(9).

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