Ast activity assay

Manufactured by Merck Group

Sourced in Germany

The AST (Aspartate Aminotransferase) Activity Assay is a laboratory test used to measure the activity of the AST enzyme in a given sample. AST is an enzyme found in various tissues, especially the liver, and its activity can provide valuable information about the health and function of these tissues.

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Lab products found in correlation

Alt activity assay, by Merck Group (3 mentions) Serum separator tube, by BD (1 mentions) Creatinine assay, by Merck Group (1 mentions) Total protein kit, by Merck Group (1 mentions) Rat insulin elisa kit, by Thermo Fisher Scientific (1 mentions) Glucose assay kit, by Merck Group (1 mentions)

Close spelling variants of this product

AST and ALT activity assays AST and ALT Activity Assay Kit Aspartate aminotransferase (AST) activity assay kit AST Activity Assay Kit ALT and AST activity assay kits AST activity

4 protocols using ast activity assay

Quantification of AST Release in Cells

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An AST activity assay (Sigma-Aldrich, St. Louis, MO) was used to quantify the release of AST into the cell-free supernatant. Cells were treated with levosimendan as previously described for MTT testing. Following this, the supernatant of each well was separately transferred into 50μl of the reaction mix. The samples were then incubated at 37°C for 2 minutes, and the initial absorbance at 450 nm was measured. Continuing the incubation at 37°C, measuring was repeated every 5 minutes until the value of the most active sample exceeded that of the highest standard. AST levels were presented as percentages of the total activity.

Brunner S.N., Bogert N.V., Schnitzbauer A.A., Juengel E., Moritz A., Werner I., Kornberger A, & Beiras-Fernandez A. (2017). Levosimendan protects human hepatocytes from ischemia-reperfusion injury. PLoS ONE, 12(11), e0187839.

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Serum Transaminase Activity Assay

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Transaminases were determined in serum using the ALT activity assay (Sigma-Aldrich, cat. no. MAK052) and the AST activity assay (Sigma-Aldrich, cat. no. MAK055) kits, following the manufacturer’s instructions.

De Sabbata G., Boisgerault F., Guarnaccia C., Iaconcig A., Bortolussi G., Collaud F., Ronzitti G., Sola M.S., Vidal P., Rouillon J., Charles S., Nicastro E., D’Antiga L., Ilyinskii P., Mingozzi F., Kishimoto T.K, & Muro A.F. (2020). Long-term correction of ornithine transcarbamylase deficiency in Spf-Ash mice with a translationally optimized AAV vector. Molecular Therapy. Methods & Clinical Development, 20, 169-180.

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Adeno-Associated Virus Liver Enzyme Assay

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8 weeks old male C57BL6/J mice were injected retro-orbitally with either saline, or 1E+12 vg (~4E+13 vg/kg) AAV9-, or MyoAAV 1A-CMV-EGFP (n=5 per group). Prior to injection, a pre-bleed sample was collected. Blood was collected using a submandibular bleed and spun down in a serum separator tube (BD). Serum samples were collected again at 14 days and 28 days post injection. ALT activity was measured using an ALT Activity Assay from Sigma (Catalog #:MAK052) based on manufacturer’s instructions for fluorometric detection using a 1:25 serum dilution. AST activity was measured with an AST Activity Assay from Sigma (Catalog #:MAK055). AST activity was also measured in duplicates according to the manufacturer’s instructions for colorimetric detection using a 1:5 serum dilution.

Tabebordbar M., Lagerborg K.A., Stanton A., King E.M., Ye S., Tellez L., Krunnfusz A., Tavakoli S., Widrick J.J., Messemer K.A., Troiano E.C., Moghadaszadeh B., Peacker B.L., Leacock K.A., Horwitz N., Beggs A.H., Wagers A.J, & Sabeti P.C. (2021). Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species. Cell, 184(19), 4919-4938.e22.

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Phthalate Exposure and Metabolic Biomarkers

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Mono(2-ethylhexyl) phthalate (CAS No. 4376-20-9, 97 % purity) and monobutyl phthalate (CAS No. 131-70-4, 97 % purity) as well as the following kits – alanine aminotransferase (ALT) activity assay (catalogue No. MAK052), aspartate aminotransferase (AST) activity assay (catalogue No. MAK055), urea assay (catalogue No. MAK266), triglyceride quantification (catalogue No. MAK006), bromocresol purple albumin assay (catalogue No. MAK125), creatinine assay (catalogue No. MAK080), total protein kit (catalogue No. TP0100), and glucose assay kit (catalog No. CBA086) – were obtained from Sigma-Aldrich (Steinheim, Germany). To measure insulin levels, we used the insulin rat ELISA Kit (Thermo-Fisher Scientific, Waltham, USA). Rat islet cell antibody (catalogue No. BG-RAT11369) was obtained from Novatein Biosciences (Woburn, MA, USA), and rat insulin antibody (catalogue No. RAB0904) from Sigma-Aldrich.

Karabulut G, & Barlas N. (2023). Endocrine Adverse Effects of Mono(2-ethylhexyl) Phthalate and Monobutyl Phthalate in Male Pubertal Rats. Archives of Industrial Hygiene and Toxicology, 73(4), 285-296.

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