Paraffin embedded prostate cancer tissue blocks were from the Vancouver Prostate Centre Tissue Bank. Patient consent was reviewed and approved by the University of British Columbia Clinical Research Ethics Board (certificate no. H09-01628). Immunohistochemical staining was conducted as previously described 56 (link) using the Ventana DiscoverXT Autostainer (Ventana Medical System) with enzyme labeled biotin streptavidin system and solvent-resistant DAB Map kit. Antibodies used in IHC include anti-FoxA1 (Abcam; ab23738), anti-SYP (Abcam; ab32127), anti-chromogranin A (Millipore; MAB5268), and anti-IL-8 (R&D systems).
Mab5268
Manufactured by R&D Systems
MAB5268 is a monoclonal antibody that binds to the human CD47 protein. CD47 is a cell surface protein involved in the regulation of phagocytosis. MAB5268 can be used for the detection and quantification of CD47 in various applications.
Automatically generated - may contain errors
2 protocols using mab5268
1
Prostate Cancer Tissue Immunohistochemistry
2
Prostate Cancer Tissue Immunohistochemistry
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Paraffin embedded prostate cancer tissue blocks were from the Vancouver Prostate Centre Tissue Bank. Patient consent was reviewed and approved by the University of British Columbia Clinical Research Ethics Board (certificate no. H09-01628). Immunohistochemical staining was conducted as previously described 56 (link) using the Ventana DiscoverXT Autostainer (Ventana Medical System) with enzyme labeled biotin streptavidin system and solvent-resistant DAB Map kit. Antibodies used in IHC include anti-FoxA1 (Abcam; ab23738), anti-SYP (Abcam; ab32127), anti-chromogranin A (Millipore; MAB5268), and anti-IL-8 (R&D systems).
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