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Diatome 45 diamond knife

Sourced in Switzerland

The DiATOME 45° diamond knife is a precision cutting tool designed for use in electron microscopy. It features a 45-degree angle diamond edge that is optimized for sectioning materials for analysis under an electron microscope. The knife is manufactured to strict quality standards to ensure consistent performance and durability.

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2 protocols using diatome 45 diamond knife

1

Transmission Electron Microscopy of PLA Films

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TEM images were obtained with an FEI Tecnai G2 20 microscope (FEI, Hillsboro, OR, USA) at the accelerating voltage of 200 kV. For the TEM sample preparation, PLA films were embedded in epoxy resin (Araldite CY212). After a 48 h curing, they were cut with ultramicrotome at 80 nm thickness (DiATOME 45° diamond knife, DiATOME ltd., Nidau, Switzerland). The thin sections floating on the knife’s water surface were deposited on carbon-coated grids and air-dried overnight.
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2

TEM Analysis of PBSu Biocomposites

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TEM studies were performed with an FEI Tecnai G2 20 microscope (FEI, Hillsboro, OR, USA) at the accelerating voltage of 200 kV. Regarding sample preparation, films of neat PBSu and its biocomposites were cut with an ultramicrotome to films of 80 nm thickness (DiATOME 45° diamond knife, DiATOME Ltd., Nidau, Switzerland). The thin sections floating on the knife’s water surface were deposited on carbon-coated grids and air-dried overnight.
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