Rapid image acquisition of entire coronal or sagittal sections was conducted using NanoZoomer whole-slide scanning microscopy (NanoZoomer Digital Pathology RS, Hamamatsu Photonics), which is capable of automatically capturing wide-field multispectral fluorescent images over entire brain sections at high resolution (Montes-Rodriguez et al., 2013 (link)). The objective was focused on the middle of the section in the z dimension and image acquisition was conducted with 40× magnification with a multi band pass filter cube (DAPI/FITC/Texas Red). Following NanoZoomer image acquisition, confocal single plane optical sections from the PC surrounding the injection site were acquired using a FV1000 laser (Olympus, America, Inc.). These confocal images were used to verify the extent of the injection site for each animal administered CTB or FG tracer.
Nanozoomer digital pathology rs
Manufactured by Hamamatsu Photonics
The NanoZoomer Digital Pathology RS is a high-performance digital slide scanner designed for pathological tissue imaging. It captures high-resolution digital images of microscope slides, enabling efficient storage, management, and analysis of pathological samples.
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Lab products found in correlation
3 protocols using nanozoomer digital pathology rs
1
Whole-Brain Fluorescent Imaging Protocol
2
Rapid Multispectral Brain Imaging
3
Prussian Blue Staining of Iron Particles
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissues used for biodistribution analysis were stained for iron particle deposition using Prussian blue. The frozen sections were fixed with 4% glutaraldehyde, washed, incubated for 20 to 30 minutes with 2% potassium ferrocyanide (Perl reagent for Prussian blue staining) in 3.7% hydrochloric acid, washed again, and counterstained with nuclear fast red. Images of the entire carotid artery sections were captured with an automated scanning microscope equipped for wide-field fluorescence (Nano Zoomer Digital Pathology RS, Hamamatsu Photonics).
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