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Control mouse igg beads

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Control mouse IgG beads are a type of lab equipment used in various research applications. They consist of agarose beads coated with mouse immunoglobulin G (IgG) antibodies, which can be used as a control in experiments involving immunoaffinity purification or other immunological techniques.

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Lab products found in correlation

Beta actin, by BD (2 mentions) Flag resin beads, by Merck Group (2 mentions) Gapdh, by Merck Group (2 mentions) Dyrk2, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) P hsf 1 s326, by Abcam (1 mentions)

Close spelling variants of this product

Control IgG (149 citations) Control mouse IgG (40 citations) Mouse IgG beads (3 citations) IgG mouse (3 citations)

2 protocols using control mouse igg beads

1

Protein Isolation and Immunoblotting Protocol

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
This was performed as we described previously [15 (link), 81 (link)]. Briefly, protein was isolated using RIPA buffer (50 mm Tris, 150 mm NaCl, 1 mm EDTA, 1% NP‐40, 1% sodium deoxycholate, 1% SDS) for immunoblotting or with IP lysis buffer (20 mm Tris, 150 mm NaCl, 1 mm EDTA, 1 mm EGTA, 1 mm sodium pyrophosphate, 1 mm β‐glycerophosphate, 1% Triton X‐100) for lysate to be used for IP. Lysates were subjected to SDS/PAGE and subjected to immunoblotting with indicated antibodies. Antibodies used for immunoblotting included p‐HSF1 (S326) (Abcam #76076, RRID:AB_1310328), HSF1 (CST #4356, RRID:AB_2120258), AKT1 (CST #2938, RRID:AB_915788), AKT2 (CST #3063, RRID:AB_2225186), AKT3 (CST #3788, RRID:AB_2242534), beta‐actin (BD Biosciences #612656, RRID:AB_2289199), GAPDH (CST #2118, RRID:AB_561053), GST (CST #2625, RRID:AB_490796), Flag (Sigma #3165, RRID:AB_259529), CDK9 (CST #2316, RRID:AB_2291505), DYRK2 (CST# 11921, RRID:AB_2797768) and GFP (CST #2956, RRID:AB_1196615). Antibodies used for IP were FLAG resin beads (Sigma #A2220) and control mouse IgG beads (Sigma #A0919).
Lu W., Omari R., Ray H., Wang J., Williams I., Jacobs C., Hockaden N., Bochman M.L, & Carpenter R.L. (2022). AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation. The Febs Journal, 289(13), 3876-3893.
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2

Immunoblotting and Immunoprecipitation Protocol for Protein Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
This was performed as we described previously [15 (link),81 (link)]. Briefly, protein was isolated using RIPA buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 1% SDS) for immunoblotting or with IP lysis buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1% Triton X-100) for lysate to be used for IP. Lysates were subjected to SDS/PAGE and subjected to immunoblotting with indicated antibodies. Antibodies used for immunoblotting included p-HSF1 (S326) (Abcam #76076, RRID:AB_1310328), HSF1 (CST #4356, RRID:AB_2120258), AKT1 (CST #2938, RRID:AB_915788), AKT2 (CST #3063, RRID:AB_2225186), AKT3 (CST #3788, RRID:AB_2242534), beta-actin (BD Biosciences #612656, RRID:AB_2289199), GAPDH (CST #2118, RRID:AB_561053), GST (CST #2625, RRID:AB_490796), Flag (Sigma #3165, RRID:AB_259529), CDK9 (CST #2316, RRID:AB_2291505), DYRK2 (CST# 11921, RRID:AB_2797768) and GFP (CST #2956, RRID:AB_1196615). Antibodies used for IP were Flag resin beads (Sigma #A2220) and control mouse IgG beads (Sigma #A0919).
Lu W., Omari R., Ray H., Wang J., Williams I., Jacobs C., Hockaden N., Bochman M.L, & Carpenter R.L. (2022). AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation. The Febs Journal, 289(13), 3876-3893.
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