Cytokines in the serum were analyzed using a Mouse G-CSF Quantikine ELISA kit (catalog #MCS00) and mouse IL-18/IL-1F4 ELISA kit (catalog #7625) according to the manufacturer’s instructions (R&D Systems, MN, USA).
Mouse il 18 il 1f4 elisa kit
Manufactured by R&D Systems
Sourced in United States
The Mouse IL-18/IL-1F4 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-18 (IL-18) levels in cell culture supernatants, serum, and plasma.
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2 protocols using mouse il 18 il 1f4 elisa kit
1
Quantifying Cytokine Levels in Serum
2
Cytokine Quantification in Murine Infection
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Blood samples were collected at different days of infection and the sera were stored at - 80°C. Samples from individual mice were diluted 10x for IL-1β quantification or non-diluted, for IL-18 detection. Cytokine levels were determined using mouse IL-18/IL-1F4 ELISA kit and mouse IL-1beta/IL-1F2 DuoSet ELISA kit (R and D Systems) according to the manufacturer's instructions.
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