HeLa cells (A1100001; Thermo Fisher Scientific, Waltham, MA, USA), BEAS-2B (CRL-9609™; American Type Culture Collection, Manassas, VA, USA), NCI-H1299 (CRL-5803™; American Type Culture Collection), HCC15 (ACC 496; Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, German), HCC95 (70095; Korean Cell Line Bank, Seoul, Korea), A549 (CRL-185™; American Type Culture Collection), PC-9 (90071810; European Collection of Authenticated Cell Cultures, Salisbury, UK), and NCI-H2009 (CRL-5911™; American Type Culture Collection) were used in this study. Cells culture was performed as described previously 18 (link).
Hcc95
Manufactured by Korean Cell Line Bank
Sourced in United States
The HCC95 is a laboratory equipment product that serves as a cell culture incubator. Its core function is to provide a controlled environment for the growth and maintenance of cell lines, ensuring optimal temperature, humidity, and gas composition for cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Hcc827, by Korean Cell Line Bank (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
H1299, by Korean Cell Line Bank (4 mentions)
Hcc1438, by Korean Cell Line Bank (4 mentions)
Hcc1588, by Korean Cell Line Bank (3 mentions)
Nci h1299, by American Type Culture Collection (2 mentions)
Beas 2b, by American Type Culture Collection (2 mentions)
Sk mes 1, by Korean Cell Line Bank (2 mentions)
Hela cell, by Thermo Fisher Scientific (1 mentions)
Nci h2009, by American Type Culture Collection (1 mentions)
Supplementmix, by PromoCell (1 mentions)
H1703, by Korean Cell Line Bank (1 mentions)
Snu 16, by American Type Culture Collection (1 mentions)
Cck 81, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Snu 638, by Korean Cell Line Bank (1 mentions)
Snu 719, by Korean Cell Line Bank (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Sk ut 1, by American Type Culture Collection (1 mentions)
Sw948, by American Type Culture Collection (1 mentions)
Snu 484, by Korean Cell Line Bank (1 mentions)
10 protocols using hcc95
1
Cell Line Characterization Protocol
2
Lung Squamous Cell Carcinoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human lung SQCC cell lines, H520 was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA), and HCC95, H1703, and SK-MES-1 were obtained from the Korean Cell Line Bank (Seoul, Korea), and cultured in RPMI 1640 medium (H520, HCC95, H1703) and Dulbecco’s modified Eagle’s medium (SK-MES-1) supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin–streptomycin (Hyclone, Logan, UT, USA). The HBEpC cells were obtained from PromoCell GmbH (Heidelberg, Germany) and cultured in an airway epithelial cell growth medium containing 2.46% SupplementMix (PromoCell GmbH). Cell culture was performed as previously described [16 (link)]. Each cell line was cultured in four biological replicates.
3
Cultivation of Non-Small Cell Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human non-small cell lung cancer cell lines (A549, H23, H226, H292, H358, H460, H522, H1299, Hcc95, Hcc827, and Hcc1438) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in RPMI-1640 medium (Hyclone, Logan, UT, USA) or DMEM/HIGH GLUCOSE (A549; Hyclone) supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies, Grand Island, NY, USA) and 1% penicillin/streptomycin solution (Hyclone). The Hcc95 cells were supplemented HEPS. The cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2.
4
Cell Culture Protocols for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human cancer cell lines SK-UT-1, SNU-16, SW948, and BT474 were obtained from the American Type Culture Collection (ATCC); HCC95, SNU-719, SNU-484 and SNU-638 from Korean Cell Line Bank (KCLB); GP2D and OE19 from the European Collection of Animal Cell Cultures (ECACC); CCK81, KNS-62, MKN45 and MKN74 from the Japanese Collection of Research Bioresources (JCRB); CJM from Riken; and QG-56 from IBL. Each cell line was cultured using the medium recommended by the suppliers and maintained in a humidified incubator at 37°C with 5% CO2, except for SW948 cells, which were cultured without CO2.
5
Culturing NSCLC and Lung Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Healthy BEAS-2B human epithelial lung and H1703 human squamous carcinoma cells (American Type Culture Collection, Manassas, VA, USA), and A549, HCC827, HCC2108, HCC95, HCC1588, and HCC1195 NSCLC cells (Korean Cell Line Bank, Seoul, Korea) were cultured as follows. BEAS-2B cells were cultured using the Bronchial Epithelial Cell Growth Medium Bullet Kit (Lonza Group Ltd., Basel, Switzerland). NSCLC cells were maintained in RPMI-1640 (WELGENE Inc., Gyeongsan-si, Korea) supplemented with 10% heat-inactivated fetal bovine serum, glutamine (2 mM), penicillin (100 U/mL), and streptomycin (100 µg/mL).
6
Lung SCC Cell Lines Knockdown of MIF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five human lung SCC cell lines, HCC‐95, HCC‐1588, SNU‐1300, SW‐900 and SK‐MES‐1, were purchased from the Korean cell line bank (Seoul, South Korea). The cell lines were cultured in RPMI‐1640 medium (Gibco, 22400‐089) supplemented with 10% fetal bovine serum (FBS, Gibco, 26140‐079), 1% penicillin‐streptomycin (Corning, 30‐002‐CI) at 37°C in 5% CO2.
To achieve knockdown of MIF, HCC‐1588 cells were transfected with small interfering RNA (siRNA). Cells were cultured in Opti‐MEM reduced serum medium (Gibco) in accordance with the manufacturer's instructions. All siRNAs were transfected using lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. Cells were harvested 72 hours after transfection and the levels of MIF mRNA were assessed by semi‐quantitative polymerase chain reaction (PCR). Western blot analysis was used to measure MIF.
To achieve knockdown of MIF, HCC‐1588 cells were transfected with small interfering RNA (siRNA). Cells were cultured in Opti‐MEM reduced serum medium (Gibco) in accordance with the manufacturer's instructions. All siRNAs were transfected using lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. Cells were harvested 72 hours after transfection and the levels of MIF mRNA were assessed by semi‐quantitative polymerase chain reaction (PCR). Western blot analysis was used to measure MIF.
7
Lung Cancer Cell Lines Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549, Calu-6, NCI-H1299, NCI-H1703, NCI-H1975, NCI-H23, NCI-H460, HCC2279, NCI-H522, A427, Calu-3, NCI-H358, HCC827 and BEAS2B were obtained from the American Type Culture Collection (Rockville, MD, USA). HCC95 and HCC1588 were obtained from the Korean Cell Line Bank (Seoul, Korea), and Normal human bronchial epithelial (NHBE) purchased from Lonza. A549, Calu-6, NCI-H1299, NCI-H1703, NCI-H1975, NCI-H23, NCI-H460, HCC2279, NCI-H522, NCI-H358, HCC827, HCC95, HCC1588, and BEAS2B were cultured with RPMI1640. Calu-3 and A427 were cultured with Dulbecco’s modified eagle medium (DMEM). NHBE was cultured with bronchial epithelial cell growth medium (BEGM) BulletKit (Lonza, Walkersville, MD, USA). All cell lines were maintained in media supplemented with 10% fetal bovine serum (FBS) and 1× penicillin-streptomycin, and cultured under standard conditions at 37 °C in a humidified atmosphere of 95% air and 5% CO2. Stock solutions of the Wee1 inhibitor MK-1775 (Selleck Chemicals, Houston, TX, USA) were dissolved in dimethyl sulfoxide (DMSO) and added to the media at the indicated concentrations (100 nM). Doxycycline (D9891) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Matrigel was purchased from Corning Inc (Corning, NY, USA).
8
Culturing Non-Small Cell Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human non-small cell lung cancer cell lines (A549, H23, H226, H292, H358, H460, H522, H1299, Hcc95, Hcc827, and Hcc1438) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in RPMI-1640 medium (Hyclone, Logan, UT, USA) or DMEM/HIGH GLUCOSE (A549; Hyclone) supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies, Grand Island, NY, USA) and 1% penicillin/streptomycin solution (Hyclone). The Hcc95 cells were supplemented HEPS. The cells were maintained at 37 o C in a humidi ed atmosphere containing 5% CO 2 .
9
Culturing Non-Small Cell Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human non-small cell lung cancer cell lines (A549, H23, H226, H292, H358, H460, H522, H1299, Hcc95, Hcc827, and Hcc1438) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in RPMI-1640 medium (Hyclone, Logan, UT, USA) or DMEM/HIGH GLUCOSE (A549; Hyclone) supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies, Grand Island, NY, USA) and 1% penicillin/streptomycin solution (Hyclone). The Hcc95 cells were supplemented HEPS. The cells were maintained at 37 o C in a humidi ed atmosphere containing 5% CO 2 .
10
Culturing Non-Small Cell Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human non-small cell lung cancer cell lines (A549, H23, H226, H292, H358, H460, H522, H1299, Hcc95, Hcc827, and Hcc1438) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in RPMI-1640 medium (Hyclone, Logan, UT, USA) or DMEM/HIGH GLUCOSE (A549; Hyclone) supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies, Grand Island, NY, USA) and 1% penicillin/streptomycin solution (Hyclone). The Hcc95 cells were supplemented HEPS. The cells were maintained at 37 o C in a humidi ed atmosphere containing 5% CO 2 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!