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3 protocols using tea polyphenols

1

Neuroprotective Effects of Natural Compounds

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Nutrients including L-tyrosine(CAS No.: 60-18-4), Vitamin B1(CAS No.: 67-03-8), Vitamin B2(CAS No.: 83-88-5), Vitamin B5(CAS No.: 137-08-6), Vitamin B6(CAS No.: 58-56-0), Vitamin B12(CAS No.: 68-19-9), Quercetin(CAS No.: 117-39-5), Resveratrol(CAS No.: 501-36-0), Tea Polyphenols(CAS No.: 84650-60-2), Genistein(CAS No.: 446-72-0), Baicalein(CAS No.: 491-67-8), Luteolin(CAS No.: 491-70-3), Proanthocyanidin(CAS No.: 4852-22-6) and Naringin(CAS No.: 10236-47-2) are all purchased from Yuanye Biotechnology (Shanghai, China); CCK8 kit and ROS kit are purchased from Beyotime Biotechnology (Shanghai, China).
Instruments includes Artificial climate box (ZRQ-400); Morris water maze (JLBehav-MWMG); Shuttle box (JLBehav-STG-4); Fluorescent dye 2′, 7′-Dichlorodihydrofluorescein diacetate (DCFH-DA, Sigma, USA).
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2

Extraction and Characterization of Bioactive Compounds

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Epigallocatechin gallate was purchased from Meryer. Procyanidin, tannic acid, and stevioside were obtained from Aladdin. Tea polyphenols and rebaudioside A were the products of Shanghai Yuanye Bio-Technology Co., Ltd. The chemicals were used as purchased without further purification, and the ultrapure water (18.2 MΩ) was used throughout the experiments.
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3

Milk-Based Fermented Product Development

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Mixed raw milk (Holsten Friesian) from different lactation periods was obtained from Zhongcheng Trading Co. Ltd. (Tangshan, China). Skim milk powder and butter for standardization were provided by Fonterra Co-Operative Group Limited (Auckland, New Zealand). Tea polyphenols (≥80%; catechins account for 74.2% of polyphenols, and epigallocatechin gallate accounts for more than 30% of catechins) were supplied by Yuanye Biotechnology Co. Ltd. (Shanghai, China). Commercial starter (a mixture of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus) was obtained from Chr. Hansen Co. Ltd. (Beijing, China) . A CML-AGE ELISA Kit and a CEL-AGE ELISA Kit were obtained from Shanghai Enzyme-linked Biotechnology Co. Ltd. (Shanghai, China) . All other chemicals were of analytical grade.
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