Dulbecco’s Modified Eagle Medium (DMEM) with high glucose was from Invitrogen. The HCC827, H292, H4006, 293T cell lines were purchased from American Type Culture Collection. Enzastaurin and erlotinib were from LC Laboratories (Woburn, MA). Go6976 and U0126 were purchased from EMD Millipore (Billerica, MA). Anti-phospho-p70S6K (Thr389), S6K, mTOR, phospho- Erk½ (Thr202/Tyr204), Erk½, (E747-A750del Specific) EGFR, EGFR, P-Akt (Ser473), P-Akt (Thr308), Akt, PKCα and ErbB3 antibodies were from Cell Signaling Technology (Danvers, MA), and anti-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Anti-EEA1 antibody was from BD Biosciences (San Jose, CA). siRNAs for PKCα and Gab1 were from Qiagen (Valencia, CA) and those for PLCγ1 were from ambion (life technologies). Dual mTOR inhibitor (KU-0063794) and Akt inhibitor (MK-2206 2HCl) were purchased from Selleckchem. All other reagents were from Sigma-Aldrich (St. Louis, MO).
Dual mtor inhibitor ku 0063794
Manufactured by Selleck Chemicals
Dual mTOR inhibitor (KU-0063794) is a laboratory compound that acts as an inhibitor of both mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). It is used in research settings to study the role of the mTOR signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle medium dmem with high glucose, by Thermo Fisher Scientific (2 mentions)
Anti actin antibody, by Merck Group (2 mentions)
Hcc827, by American Type Culture Collection (2 mentions)
U0126, by Merck Group (2 mentions)
H4006, by American Type Culture Collection (2 mentions)
Go6976, by Merck Group (2 mentions)
Anti eea1 antibody, by BD (2 mentions)
Sirnas for pkcα and gab1, by Qiagen (2 mentions)
Those for plcγ1, by Thermo Fisher Scientific (2 mentions)
Akt inhibitor mk 2206 2hcl, by Selleck Chemicals (2 mentions)
Erbb3, by Cell Signaling Technology (2 mentions)
Erlotinib, by LC Laboratories (2 mentions)
Enzastaurin, by LC Laboratories (2 mentions)
2 protocols using dual mtor inhibitor ku 0063794
1
Investigating EGFR Signaling Pathways
2
Investigating EGFR Signaling Pathways
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Dulbecco’s Modified Eagle Medium (DMEM) with high glucose was from Invitrogen. The HCC827, H292, H4006, 293T cell lines were purchased from American Type Culture Collection. Enzastaurin and erlotinib were from LC Laboratories (Woburn, MA). Go6976 and U0126 were purchased from EMD Millipore (Billerica, MA). Anti-phospho-p70S6K (Thr389), S6K, mTOR, phospho- Erk½ (Thr202/Tyr204), Erk½, (E747-A750del Specific) EGFR, EGFR, P-Akt (Ser473), P-Akt (Thr308), Akt, PKCα and ErbB3 antibodies were from Cell Signaling Technology (Danvers, MA), and anti-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Anti-EEA1 antibody was from BD Biosciences (San Jose, CA). siRNAs for PKCα and Gab1 were from Qiagen (Valencia, CA) and those for PLCγ1 were from ambion (life technologies). Dual mTOR inhibitor (KU-0063794) and Akt inhibitor (MK-2206 2HCl) were purchased from Selleckchem. All other reagents were from Sigma-Aldrich (St. Louis, MO).
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