Jsm 35 scanning electron microscope

To visualize the shape and properties of newly synthesized AgNPs (naked AgNPs, AgNP-TBA, and AgNP-MUA), the scanning electron microscopy (SEM) technique was used. Tested samples were deposited on cleaned silicon p-type (100) plates. Then, images of deposits were obtained in the secondary electron mode at 25 kV energy using a JSM-35 scanning electron microscope (JEOL, 3-1-2 Musashino, Akishima, Tokyo, Japan). AgNPs were studied in the as-prepared pristine state and, sometimes, images of large particles were slightly distorted as a result of their charging under electron beam irradiation.

Trichophyton tonsurans strain RCPFF 214/898, isolated from a human patient with onychomycosis, was obtained from the Russian Collection of Pathogenic Fungi, St. Petersburg (Russia). Identity was confirmed by sequencing the rDNA ITS locus. The strain was grown on solid CzA at 28ºС and investigated after 5, 10, 20 and 30 days of cultivation. Colonies were photographed with an Olympus BX 51 camera. For scanning electron microscopy (SEM), small parts of fungal colonies were fixed in 3% glutaraldehyde (in cacodylate buffer, pH 7.2) for 3 h, post-fixed overnight in 1% osmium tetroxide in the same buffer, dehydrated by ethanol series (30%→50%→70%), critical-point dried (HCP-2) for 15 min, coated with gold and observed in a JSM 35 scanning electron microscope (Jeol, Tokyo, Japan).
For transmission electron microscopy (TEM), blocks of nutrient medium with parts of fungal colonies were fixed during the 3 h in 3% glutaraldehyde and post-fixed for 10 h in 1% osmium tetroxide. Subsequently, samples were dehydrated through an ethanol and acetone series and embedded in epon-araldite epoxy resin. Ultrathin sections were cut with an Ultratome 2088 (LKB, Bromma, Sweden), stained with uranyl acetate and lead citrate and were investigated under a TEM Jem 100 SX (Jeol).