Log In Sign up
The largest database of trusted experimental protocols

Anti p107

Manufactured by Proteintech
Visit Supplier

Anti-P107 is a lab equipment product manufactured by Proteintech. It is a primary antibody used for the detection and analysis of the P107 protein in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Proteinase inhibitors cocktail, by Roche (2 mentions) Anti e2f3, by ABclonal (2 mentions) Anti gsk3b, by ABclonal (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Ripa lysis buffer, by Beyotime (2 mentions) Enhanced chemi luminescence (ecl), by PerkinElmer (2 mentions)

Close spelling variants of this product

Anti-TM

2 protocols using anti p107

1

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extracts from tissues were prepared using RIPA lysis buffer (Beyotime). Tissues or cells were harvested and lysed in RIPA Buffer (50mM Tris (pH 7.4), 150mM NaCl, 1% Triton X-100, 1% sodium deoxycholate) containing proteinase inhibitors cocktail (Roche) on ice for 40 minutes. Lysates were centrifuged 40 minutes at 4°C at maximum speed (20000 rcf.) and resulting protein extracts separated by SDS–PAGE (12%). After transfer of proteins to PVDF membranes (Bio-rad), membranes were blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBS-T) for 1 hr at room temperature, followed by two washes with TBS-T. Membranes were incubated overnight at 4°C with primary antibodies followed by three 10-min washes in TBS-T, exposure to HRP-conjugated secondary antibody for 1 hr at room temperature, and 3 washes with TBS-T. Detection of HRP conjugated secondary antibody was performed with ECL (PerkinElmer). Antibodies used in supplemental experiment were anti-E2F3 (ABclonal Technology, A8811), anti-GSK3B (ABclonal Technology, A0479), Anti-P107 (Proteintech, 13354–1-AP).
Lin K., Qiang W., Zhu M., Ding Y., Shi Q., Chen X., Zsiros E., Wang K., Yang X., Kurita T, & Xu E.Y. (2019). Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports, 26(9), 2434-2450.e6.
+ Open protocol
+ Expand
2

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extracts from tissues were prepared using RIPA lysis buffer (Beyotime). Tissues or cells were harvested and lysed in RIPA Buffer (50mM Tris (pH 7.4), 150mM NaCl, 1% Triton X-100, 1% sodium deoxycholate) containing proteinase inhibitors cocktail (Roche) on ice for 40 minutes. Lysates were centrifuged 40 minutes at 4°C at maximum speed (20000 rcf.) and resulting protein extracts separated by SDS–PAGE (12%). After transfer of proteins to PVDF membranes (Bio-rad), membranes were blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBS-T) for 1 hr at room temperature, followed by two washes with TBS-T. Membranes were incubated overnight at 4°C with primary antibodies followed by three 10-min washes in TBS-T, exposure to HRP-conjugated secondary antibody for 1 hr at room temperature, and 3 washes with TBS-T. Detection of HRP conjugated secondary antibody was performed with ECL (PerkinElmer). Antibodies used in supplemental experiment were anti-E2F3 (ABclonal Technology, A8811), anti-GSK3B (ABclonal Technology, A0479), Anti-P107 (Proteintech, 13354–1-AP).
Lin K., Qiang W., Zhu M., Ding Y., Shi Q., Chen X., Zsiros E., Wang K., Yang X., Kurita T, & Xu E.Y. (2019). Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell reports, 26(9), 2434-2450.e6.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!