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Sildenafil

Manufactured by Cayman Chemical
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Sildenafil is a chemical compound commonly used in laboratory research. It is a phosphodiesterase type 5 (PDE5) inhibitor, which means it inhibits the activity of the PDE5 enzyme. This enzyme plays a role in the regulation of certain physiological processes. Sildenafil is often utilized in scientific investigations, but its specific applications and intended uses are not provided in this factual description.

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Lab products found in correlation

Nifedipine, by Merck Group (2 mentions) Celecoxib, by Cayman Chemical (2 mentions) Adenosine, by Merck Group (1 mentions) Isoproterenol, by Merck Group (1 mentions) L arginine, by Merck Group (1 mentions) Diclofenac, by Cayman Chemical (1 mentions) Tamsulosin hydrochloride, by Merck Group (1 mentions) Butoxamine, by Merck Group (1 mentions) Mirabegron, by Cayman Chemical (1 mentions) Vardenafil, by Cayman Chemical (1 mentions) Y 27632 dihydrochloride, by R&D Systems (1 mentions) Rolipram, by Cayman Chemical (1 mentions) Sterile cell culture grade dmso, by Merck Group (1 mentions) L arginine, by Cayman Chemical (1 mentions) Captopril, by Merck Group (1 mentions) Memantine, by Merck Group (1 mentions) Zileuton, by Cayman Chemical (1 mentions) Skf96365, by Merck Group (1 mentions) Acetaminophen, by Merck Group (1 mentions) Phenytoin, by Merck Group (1 mentions)

6 protocols using sildenafil

1

Preparation of Compound Stock Solutions

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100 mM stock solutions of all screened compounds were prepared in individual, sterile amber vials. All stock solutions were dissolved in sterile DMSO, except L-arginine, which was solubilized using dH2O, due to solubility constraints. Sterile cell-culture grade DMSO, tamsulosin hydrochloride, nifedipine, isoproterenol, butoxamine, adenosine and L-arginine were obtained from Sigma Aldrich. PGE1, PGE2, rolipram, L-arginine, ondansetron, celecoxib, diclofenac, mirabegron, sildenafil, atropine, vardenafil and tiotropium were obtained from Cayman Chemical. Y-27632 dihydrochloride was obtained from R&D Systems. Stock solutions when unused were stored at −20 °C
Lee C.X., Cheah J.H., Soule C.K., Ding H., Whittaker C.A., Karhohs K., Burds A.A., Subramanyam K.S., Carpenter A.E., Eisner B.H, & Cima M.J. (2019). Identification and local delivery of vasodilators for the reduction of ureteral contractions. Nature biomedical engineering, 4(1), 28-39.
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2

Pharmacological Compounds for Research

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SKF 96365, captopril, cyproheptidine, scopolamine, tert-butylhydroquinone (tBHQ), and memantine (Sigma Aldrich; St. Louis, MO); sildenafil, zileuton, and CP-80633 (Cayman Chemical; Ann Arbor, MI); valproic acid (VPA; Qualitest Pharmaceuticals; Huntsville, AL), vigabatrin (Lundbeck; Deerfield, IL), metformin (Ranbaxy Laboratories Inc.; Jacksonville, FL), Szeto-Schiller-31 (SS-31; GenScript; Piscataway, NJ); HC-030031 (Hydra Biosciences; Cambridge, MA); ambrisentan (Gilead; Foster City, CA), and RR (Acros Organics; Pittsburgh, PA).
Holmes W.W., Keyser B.M., Paradiso D.C., Ray R., Andres D.K., Benton B.J., Rothwell C.C., Hoard-Fruchey H.M., Dillman J.F., Sciuto A.M, & Anderson D.R. (2015). Conceptual Approaches for Treatment of Phosgene Inhalation-Induced Lung Injury. Toxicology letters, 244, 8-20.
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3

Evaluating Anti-seizure Drugs in Rats

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Rats implanted with ventral hippocampal electrodes and dorsal hippocampal optodes were used in these studies. Rats were reused for several drug experiments allowing at least two days between drug treatments. Celecoxib, SC-560, ibuprofen, chelerythrine chloride, milrinone, sildenafil, SKA-31, CAY-10526, seratrodast, ozagrel, paxilline, 2-APB, levetiracetam, and topiramate were obtained from Cayman Chemicals (Ann Arbor, MI). Acetaminophen, nifedipine, bumetanide, ethosuximide, phenytoin, and valproic acid were obtained from Sigma-Aldrich. Lamotrigine was obtained from SelleckChem (Houston, TX). Fasudil was obtained from LC laboratories (Woburn, MA) and phenobarbital was obtained from Strathcona Prescription Center (Canada). Lipophilic drugs were dissolved in 100% DMSO, while hydrophilic drugs were dissolved in saline and injected 30 min prior to seizure induction. The seizure duration and severity of hypoxia (area below 10 mmHg) were compared across kindle (seizure without injection), vehicle-, and drug-treated groups using a within-subject ANOVA and follow-up t-test between vehicle- and drug-treated groups.
Farrell J.S., Gaxiola-Valdez I., Wolff M.D., David L.S., Dika H.I., Geeraert B.L., Rachel Wang X., Singh S., Spanswick S.C., Dunn J.F., Antle M.C., Federico P, & Teskey G.C. (2016). Postictal behavioural impairments are due to a severe prolonged hypoperfusion/hypoxia event that is COX-2 dependent. eLife, 5, e19352.
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4

Pharmacological Regulation of Smooth Muscle

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STO-609 and GSK1901320 (referred to as GSKi) were synthesized at the Duke University Small Molecule Synthesis Facility. Ionomycin (catalog #10004974) was obtained from Cayman Chemicals and solubilized in DMSO at room temperature, sterile filtered (0.2 μmol/L), and stored at −20°C. The pan-PDE inhibitor, IBMX (catalog #BML-PD140) and PDE1-specific inhibitor, vinpocetine (catalog #BML-PD185) were purchased from Enzo Life Sciences. Sildenafil (catalog #10008671) was obtained from Cayman Chemicals. The nitric oxide synthase (NOS) inhibitors, Nω-Nitro-L-arginine (L-NNA; catalog #N5501) and Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME; catalog #N5751) were both obtained from Millipore Sigma and solubilized in 1 mol/L HCL and dH2O, respectively. To stain filamentous actin, rhodamine-phalloidin (catalog #R415) was purchased from Thermo Fisher Scientific, dissolved in methanol, and stored at −20°C. The small-molecule PKG inhibitors, RKRARKE (catalog #370654) and KT5823 (catalog #K1388) were both obtained from Millipore Sigma.
Mukherjee D., Previs R.A., Haines C., Al Abo M., Juras P.K., Strickland K.C., Chakraborty B., Artham S., Whitaker R.S., Hebert K., Fontenot J., Patierno S.R., Freedman J.A., Lau F.H., Burow M.E., Chang C.Y, & McDonnell D.P. (2023). Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis. Cancer Research, 83(17), 2889-2907.
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5

Rodent Models of Pulmonary Hypertension

Cited 1 time
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Animal studies were approved by the Institutional Animal Care and Use Committee at Acceleron Pharma Inc., a subsidiary of Merck & Co., Inc., Kenilworth, NJ, USA, in accordance with ARRIVE guidelines. Adult male Sprague–Dawley (SD) and Wistar (WI) rats (150–180 gm) (Envigo, Indianapolis, IN) were used as SuHxNx and MCT rat models, respectively. The SuHxNx model was established by a single subcutaneous injection of vascular endothelial growth factor receptor antagonist semaxanib (SU5416, 20 mg/kg; Cayman Chemical) with immediate onset of exposure to normobaric hypoxia (10% O2) for 3 weeks followed by normoxia (21% O2) for 6 weeks as described34 (link),35 (link),70 (link). Sildenafil was obtained from Cayman Chemical. The MCT model was established by a single subcutaneous injection of MCT (60 mg/kg, Torris) followed by 4 week exposure to normoxia. Bmpr2+/R899X mice were generated as a model of BMPR2 haploinsufficiency as described44 (link).
Joshi S.R., Liu J., Bloom T., Karaca Atabay E., Kuo T.H., Lee M., Belcheva E., Spaits M., Grenha R., Maguire M.C., Frost J.L., Wang K., Briscoe S.D., Alexander M.J., Herrin B.R., Castonguay R., Pearsall R.S., Andre P., Yu P.B., Kumar R, & Li G. (2022). Sotatercept analog suppresses inflammation to reverse experimental pulmonary arterial hypertension. Scientific Reports, 12, 7803.
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6

Cyclic Nucleotide Signaling Modulators

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The following drugs were used: ANP and CNP (Tocris, Bristol, UK), DEA/NO (Axxora, Ann Arbor, MI, USA), glutamate (Sigma, St. Louis, MO, USA), IBMX (Sigma), Vinpocetine (Cayman, Ann Arbor, MI, USA), Bay 60-7550 (Cayman), EHNA (Axxora), Milrinone (Sigma), Sildenafil (Cayman), Zaprinast (Santa Cruz, Dallas, TX, USA), 8-Br-cGMP (Biolog, Bremen, Germany), and 8-pCPT-cGMP (Biolog).
Paolillo M., Peters S., Schramm A., Schlossmann J, & Feil R. (2018). Real-Time Imaging Reveals Augmentation of Glutamate-Induced Ca2+ Transients by the NO-cGMP Pathway in Cerebellar Granule Neurons. International Journal of Molecular Sciences, 19(8), 2185.
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