F. nucleatum (ATCC 25586), P. anaerobius (ATCC 27337), and P. micra (ATCC 33270) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). All bacteria were cultured at 37°C in Anaerobe basal broth (CM0957, OXOID, Thermo Fisher Scientific, West Palm Beach, FL, USA) in an anaerobic jar (Hardy Diagnostics, Santa Maria, CA, USA). Anaerobic conditions were maintained using AnaeroGen packets (Thermo Fisher Scientific).
Anaerobic jar
Manufactured by Hardy Diagnostics
Sourced in United States
The Anaerobic Jar is a laboratory equipment used to create and maintain an anaerobic environment, which is essential for the growth and cultivation of anaerobic microorganisms. The jar provides a controlled, oxygen-depleted atmosphere to support the survival and proliferation of anaerobic bacteria and other anaerobic organisms during microbial testing and analysis.
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Lab products found in correlation
3 protocols using anaerobic jar
1
Culturing Anaerobic Oral Bacteria
2
Isolation and Characterization of Parvimonas micra
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Fecal samples from CRC patients with high relative abundance of P. micra were used for bacterial isolation. Samples were spread on horse blood agar plates and incubated in anaerobic chamber. The identity of the candidate colony (P. micra strain 512) was determined by gram staining and amplification of 16 S rRNA gene using universal primers targeting hypervariable regions V1-V4 and V6 of the 16 S rRNA gene, with further confirmation by P. micra-specific primers and Sanger sequencing. The bacteria were cultured in anaerobe basal broth (CM0957; Thermo Fisher Scientific) in anaerobic jar (Hardy Diagnostics, Santa Maria, CA). The anaerobic condition was created by the application of Anaerogen (AN0035; Thermo Fisher Scientific). Another strain (P. micra Smith) was bought from American Type Culture Collection (33270,ATCC, Manassas, VA).
3
Anaerobic Cultivation and Comparison of P. anaerobius
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P anaerobius (ATCC 27337) was purchased from American Type Culture Collection (Manassas, VA). The bacteria were maintained in anaerobe basal broth (CM0972; Thermo Fisher Scientific, West Palm Beach, FL) in anaerobic jar (Hardy Diagnostics, Santa Maria, CA). The anaerobic condition was created by the usage of Anaerogen (AN0035; Thermo Fisher Scientific, Waltham, MA). A nonpathogenic human commensal intestinal bacteria, E coli strain MG1655 22 was used as a control for P anaerobius. E coli MG1655 was cultured in lysogeny broth under aerobic condition.
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