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A1 inverted microscope

Manufactured by Nikon
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The Nikon A1 inverted microscope is a high-performance laboratory instrument designed for advanced imaging applications. It features a compact and modular design, allowing for customization to meet the specific needs of various research fields. The A1 inverted microscope provides users with a stable and reliable platform for delivering high-quality, detailed images of samples under examination.

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Lab products found in correlation

Growth factor reduced matrigel, by BD (1 mentions) Rat igg2a isotype antibody clone ebr2a, by Thermo Fisher Scientific (1 mentions) Treg isolation kit, by Miltenyi Biotec (1 mentions) Nis element, by Nikon (1 mentions)

Close spelling variants of this product

Inverted Ti-E A1 confocal microscope A1 LUN-V inverted microscope system A1 LUNA inverted microscope A1-RS inverted laser scanning confocal microscope Ti-E scanning laser confocal inverted microscope (A1) Nikon A1 confocal/Eclipse Ti inverted microscope system A1-Rs inverted laser scanning microscope A1+ inverted confocal laser scanning microscope A1 inverted confocal microscope A1 Resonance Plus inverted microscope

5 protocols using a1 inverted microscope

1

Quantifying JHMV Infection in NPCs

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To determine JHMV infectivity of cultured NPCs, cells were plated on reduced growth factor-matrigel (BD Biosciences) coated chamber slides. NPCs were infected with JHMV (moi=0. 4) for 18 hours and fixed at 72 hours p.i with 4% PFA for 20 minutes at room temperature. Immunofluorescence was performed as described previously (Whitman et al., 2009) using antibodies specific for rabbit monoclonal anti-Sox 2 (abcam, 1:100 dilution) or monoclonal antibody specific for JHMV spike protein (Plaisted et al., 2014 (link)) and Alexa Flour 594 goat anti-rabbit secondary antibody (Life technologies). Slides were then imaged using a Nikon A1 inverted microscope.
Mangale V., Marro B.S., Plaisted W.C., Walsh C.M, & Lane T.E. (2017). Neural precursor cells derived from induced pluripotent stem cells exhibit reduced susceptibility to infection with a neurotropic coronavirus. Virology, 511, 49-55.
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2

Quantitative Analysis of Spermatid Maturation

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Imaging was conducted on a confocal Nikon A1 Inverted microscope. Each testis was scored for nuclei without associated cones (condensed nuclei that did not co-localize with actin cones), nuclei with cones (actin cones that were in register and colocalized with nuclear bundles), migrating cones (cone groups that were in register and did not colocalize with the spermatid nuclei bundle) and waste bags (rounded waste bags near the apical end of the testis).
Pravder H.D., Grabowska D., Roychoudhury K., Zhang B., Frank D., Zakrzewski P., Lenartowska M, & Miller K.G. (2022). PFTAIRE Kinase L63 Interactor 1A (Pif1A Protein) Is Required for Actin Cone Movement during Spermatid Individualization in Drosophila melanogaster. International Journal of Molecular Sciences, 23(6), 3011.
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3

Lumbrical Muscle Cryosectioning and Immunofluorescence

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Lumbrical muscles were embedded in O.C.T. compound, frozen in liquid nitrogen cooled isopentane for 1 minute and stored at −80 °C. 10 µm cryosections were taken from the mid-portion of the muscle, cut at −20 °C with a cryotome and applied to slides. Immunofluorescent staining was carried out on frozen lumbrical sections. Primary and secondary antibodies are described as below. Fluorescent images were taken by Nikon A1 inverted microscope.
Su Y., Ahn B., Macpherson P.C., Ranjit R., Claflin D.R., Van Remmen H, & Brooks S.V. (2021). Transgenic expression of SOD1 specifically in neurons of Sod1 deficient mice prevents defects in muscle mitochondrial function and calcium handling. Free radical biology & medicine, 165, 299-311.
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4

Microscopy Imaging Protocol for Whole Sections

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Prepared slides were imaged with Nikon A1 inverted microscope using a 10X, 20X or 63X objective. Whole section scans were acquired with either the Nikon A1 microscope with 10X objective, or the Odyssey-M whole slide imaging functionality. Whole section scans were imported into adobe photoshop for downsizing, cutting, auto-color balancing, and auto-brightness adjustments. Edited images were imported into adobe illustrator for arrangement and final presentation in figures.
Choi S., Tittle T., Garcia-Prada D., Kordower J., Melki R, & Killinger B. (2024). Alpha-synuclein aggregates are phosphatase resistant. bioRxiv.
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5

Imaging pDC-Treg Interactions in vitro

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Splenic pDCs were plated at 5 × 105 cells per dish in 35-mm collagen-coated glass-bottom culture dishes (MatTek) in 10% FBS complete RPMI. Cells were pulsed with PBS or low-dose or high-dose gp96 for 18 h. CD4+CD25+ Tregs or CD4+CD25− Tconv were isolated from spleen using Treg isolation kit (Miltenyi) and labelled with CellTracker Red dye before addition to the culture and imaging. Nrp1 blocking monoclonal antibody (clone 761704; RnD Systems) or rat IgG2a isotype antibody (clone eBR2a; eBioscience) were added 2 h before imaging at a final concentration of 10 μg ml−1. Antibody clones and concentrations were based on previously established protocols38 (link). Cells were imaged on a Nikon A1 inverted microscope (Nikon) using a 40X objective. Images were captured every 5 min for a total of 1 or 2 h. Videos were analysed using NIS Elements (Nikon) and ImageJ66 with Manual Tracking plugin. For interaction time analysis, total contact duration per Treg/Tconv detected from videos was calculated based on the number of frames in which a Treg/Tconv came in contact with a pDC.
Kinner-Bibeau L.B., Sedlacek A.L., Messmer M.N., Watkins S.C, & Binder R.J. (2017). HSPs drive dichotomous T-cell immune responses via DNA methylome remodelling in antigen presenting cells. Nature Communications, 8, 15648.
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