Primescript rt master mix kit

Total RNA was extracted from cells with TRIzol reagent, and then cDNA was synthesized using the PrimeScript RT Master Mix kit (QIAGEN, Germany). Quantitative reverse transcription-polymerase chain reaction using ChamQTM Universal SYBR^® qPCR Master Mix (Vazyme, China). The PCRs were then performed using the 7500 Real-Time PCR System (Applied Biosystems, USA) with the primers as follows: HER-2 forward, 5′-AGCCGCGAGCACCCAAGT-3′, reverse, 5′-TTGGTGGGCAGGTAGGTGAGTT-3ʹ. GAPDH: forward 5′-GATGACTACCGTCCACTCC-3′ and reverse 5′-ACTCTGAAAGCCATACCG-3′. NCI-N87 cells were used as a mock control 3.5×10⁵ cells in 12-well plates (NEST, China) until approximately 90% confluent.

Total RNA was extracted from trizol reagent according to the manufacturer’s instructions (Vazyme, China), and then cDNA was synthesized using PrimeScript RT Master Mix kit (QIAGEN, Germany). The expression of target genes was standardized to the expression of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). And the following primers were used to detect the expression of HER-3 and GAPDH: HER-3 forward, 5′-AAGCTCTACGAGAGGTGTGA-3′, reverse, 5′-TGGGCAATGGTAGAGTAGAG-3ʹ. GAPDH: forward 5′-GATGACTACCGTCCACTCC-3′ and reverse 5′-ACTCTGAAAGCCATACCG-3′.