Photo paint 2019

The G-banding was performed for ten Columbidae species with a combination of DAPI and propidium iodide [19 (link)] in order to detect intrachromosomal rearrangements not observed by chromosome painting. Images were captured by an Olympus BX61 epifluorescence microscope with a cooled CCD camera and SmartCapture (Digital Scientific UK) system. Afterward, the images were converted to grayscale using Corel Photo-Paint 2019.

The long-term cell survival after treatments was determined by clonogenic assay. PANC-1 cells were seeded in a transparent 6-well cell culture plate (VWR) (density: 750 cells/well). After seeding, cells were incubated for 72 h before the treatment. Cells were treated with the compounds at 10 µM for 24 or 72 h. Then, the medium was removed, and the cells were washed with phosphate-buffered saline (PBS). The cells formerly treated for 24 or 72 h were further incubated in their corresponding medium for 5 or 3 days, respectively. Thereafter, the medium was removed, and the cells were washed with PBS and were fixed with 4% paraformaldehyde for 10 min. After fixation, cells were washed with PBS, and 0.5% w/v crystal violet (CV) solution was added. After 1 h, the CV solution was removed, and cells were washed thoroughly with water. Images were created by Corel Photo-Paint 2019.

Whole embryos were photographed using the Leica Application Suite (LAS) 4.6 software with a M165 FC stereomicroscope and a DFC450 C camera (both from Leica). Images of histological sections were acquired with the KS400 3.0 software using an Axioskop 2 MOT microscope and an AxioCam HR digital camera (Carl Zeiss, Göttingen, Germany). Following background and shading corrections in the respective imaging software, digital photographs were cropped and adjusted for brightness, color balance, and sharpness in Photo-Paint 2019 (Corel, Unterschleißheim, Germany). All image adjustments were carried out on the entire images without changing, removing or inserting specific features within the photographs. All figures and lettering were composed using CorelDraw 2019 (Corel). 3D reconstructions were created in the reconstruction and modeling software Free-D 1.15 (Andrey and Maurin, 2005 (link)).