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Turbo pumped thermal evaporators model k975x

Manufactured by Quorum Technologies
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The Turbo-Pumped Thermal Evaporators model K975X is a laboratory equipment designed for the deposition of thin films. It utilizes a turbo-molecular pump to maintain a high vacuum environment during the evaporation process. The core function of this equipment is to vaporize and deposit materials onto a substrate in a controlled manner.

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Lab products found in correlation

Sputter coater model k575x, by Quorum Technologies (2 mentions) Auriga, by Zeiss (1 mentions) Sputter coater, by Quorum Technologies (1 mentions)

Close spelling variants of this product

K975X Turbo-Pumped Thermal Evaporator

5 protocols using turbo pumped thermal evaporators model k975x

1

Density-dependent Scanning Electron Microscopy

Cited 1 time
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Density-dependent scanning electron microscopy was performed at University College London as previously described (8 (link)). Briefly, human carotid artery or aortic valve sections on glass slides were secured to aluminum sample holders with carbon tape, and silver paint was applied to the area immediately surrounding each sample. Samples were then coated with 5-nm carbon (Quorum Technologies Turbo-Pumped Thermal Evaporators model K975X, Lewes, UK). Following coating, samples were imaged on a scanning electron microscope (SEM; Zeiss VP), operated at 10 kV, and equipped with both an inlens detector that recorded secondary electrons and a backscatter electron detector. Images were obtained by imaging a region in inlens mode and subsequently imaging the same region in backscatter mode. Adobe Photoshop CC 2018 was used on stacked images, and the inlens image was assigned to the green channel whereas the backscatter image was assigned to the red channel.
Rogers M.A., Buffolo F., Schlotter F., Atkins S.K., Lee L.H., Halu A., Blaser M.C., Tsolaki E., Higashi H., Luther K., Daaboul G., Bouten C.V., Body S.C., Singh S.A., Bertazzo S., Libby P., Aikawa M, & Aikawa E. (2020). Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Science Advances, 6(38), eabb1244.
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2

Visualizing Extracellular Vesicles by SEM

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For SEM imaging, typically 200–400 μL of EVs were mixed with uranyl acetate (0.05 wt%) or hemin (final 1 mg mL−1 w/v) for 10 min at room temperature to allow labeling. EVs were purified by size-exclusion chromatography (SEC), characterized and loaded into hydrogels as described above. Hydrogels containing labeled EVs, native EVs, or PBS control gels were dehydrated with increasing amounts of methanol (10–100% v/v in water), for 1 h at each step and air dried, snap frozen in liquid nitrogen, and cut with a razor blade. Samples were attached to aluminum stubs with carbon tape, silver paint was spread on the sample sides, and coated with 5 nm carbon (Quorum Technologies Turbo-Pumped Thermal Evaporators model K975X). Gels were imaged by SEM (Zeiss Auriga) operated at 5 kV, equipped to record in-lens and secondary electrons, and in backscatter mode. The density-dependent color SEM analysis was executed as described previously,[17 (link)] briefly the in-lens or secondary electron image was assigned to the green channel while the backscattering signal was assigned to the red channel; images were stacked using ImageJ.
Fuhrmann G., Chandrawati R., Parmar P.A., Keane T.J., Maynard S.A., Bertazzo S, & Stevens M.M. (2018). Engineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy. Advanced materials (Deerfield Beach, Fla.), 30(15), e1706616.
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3

Density-Dependent Color SEM Imaging

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For density-dependent color scanning electron microscopy (DDC-SEM) imaging, histologically sectioned samples, intact collagen hydrogels, or collagenase isolated hydrogel components on a glass slide were secured to an aluminum sample holder with carbon tape, and silver paint was applied to the area immediately surrounding each sample, which was then coated with 5nm carbon (Quorum Technologies Turbo-Pumped Thermal Evaporators model K975X) and 5nm chromium in a sputter coater (Quorum Technologies Sputter Coater model K575X). Following the coating procedure, samples were imaged by an SEM (Gemini 1525 FEGSEM), operated at 10 kV. The instrument was equipped with both an inlens detector that recorded secondary electrons, and a backscatter electron detector. The DDC-SEM images were obtained by imaging a region in inlens mode and subsequently imaging the same region in backscatter mode. Using ImageJ software, both images were stacked and the inlens image was assigned to the green channel whereas the backscatter image was assigned to the red channel.
Hutcheson J.D., Goettsch C., Bertazzo S., Maldonado N., Ruiz J.L., Goh W., Yabusaki K., Faits T., Bouten C., Franck G., Quillard T., Libby P., Aikawa M., Weinbaum S, & Aikawa E. (2016). Genesis and growth of extracellular vesicle-derived microcalcification in atherosclerotic plaques. Nature materials, 15(3), 335-343.
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4

Density-Dependent Color SEM Imaging

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For density-dependent color scanning electron microscopy (DDC-SEM) imaging, histologically sectioned samples, intact collagen hydrogels, or collagenase isolated hydrogel components on a glass slide were secured to an aluminum sample holder with carbon tape, and silver paint was applied to the area immediately surrounding each sample, which was then coated with 5nm carbon (Quorum Technologies Turbo-Pumped Thermal Evaporators model K975X) and 5nm chromium in a sputter coater (Quorum Technologies Sputter Coater model K575X). Following the coating procedure, samples were imaged by an SEM (Gemini 1525 FEGSEM), operated at 10 kV. The instrument was equipped with both an inlens detector that recorded secondary electrons, and a backscatter electron detector. The DDC-SEM images were obtained by imaging a region in inlens mode and subsequently imaging the same region in backscatter mode. Using ImageJ software, both images were stacked and the inlens image was assigned to the green channel whereas the backscatter image was assigned to the red channel.
Hutcheson J.D., Goettsch C., Bertazzo S., Maldonado N., Ruiz J.L., Goh W., Yabusaki K., Faits T., Bouten C., Franck G., Quillard T., Libby P., Aikawa M., Weinbaum S, & Aikawa E. (2016). Genesis and growth of extracellular vesicle-derived microcalcification in atherosclerotic plaques. Nature materials, 15(3), 335-343.
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5

Sample Preparation for Electron Microscopy

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Samples were secured to an aluminium sample holder with carbon tape and carbon paste, which was then coated with 5 nm carbon (Quorum Technologies Turbo-Pumped Thermal Evaporators model K975X) and 5 nm chromium in a Sputter Coater (Quorum Technologies Sputter Coater model K575X).
Bertazzo S., Maidment S.C., Kallepitis C., Fearn S., Stevens M.M, & Xie H.N. (2015). Fibres and cellular structures preserved in 75-million–year-old dinosaur specimens. Nature Communications, 6, 7352.
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