Albumin content, as a measure to quantify the increased permeability of the alveolar-capillary barrier, and the activity of the intracellular enzyme lactate dehydrogenase, an indicator of cellular cytotoxicity, were determined in BAL samples [22 (link),23 (link)]. Albumin content was determined colorimetrically based on albumin binding to bromocresol green using a Wiener-Lab albumin diagnostic kit. LDH activity, expressed as units per liter of BAL, was determined by measuring the formation of the reduced form of NAD+ using Wiener’s reagents and procedures (Wiener-Lab).
Wiener s reagents and procedures
Manufactured by Wiener Lab
Wiener's reagents and procedures are laboratory products designed for use in various scientific and medical applications. They provide standardized, reliable tools for researchers and healthcare professionals to conduct necessary analyses and testing. The core function of these reagents and procedures is to enable precise, accurate, and reproducible laboratory work across a range of disciplines. No further interpretation or extrapolation on the intended use of these products is provided.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using wiener s reagents and procedures
1
Quantifying Alveolar-Capillary Barrier and Cytotoxicity
2
Quantifying Lung Barrier Integrity and Cytotoxicity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The albumin content in BAL was quantified as an indicator of the increased permeability of the bronchoalveolar capillary barrier [21 (link)]. In addition, lactate dehydrogenase (LDH) activity was quantified as an indicator of general cytotoxicity [22 (link)]. Albumin content was determined colorimetrically using a diagnostic kit (Wiener Lab, Buenos Aires, Argentina) that is based on the binding of albumin to bromocresol green. LDH activity, expressed as units per litre of BAL fluid, was determined using Wiener’s reagents and procedures (Wiener Lab), which measure the formation of the reduced form of nicotinamide adenine dinucleotide (NAD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!