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Rneasy 96 mini kit

Manufactured by Qiagen
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Sourced in Germany

The RNeasy 96 Mini Kit is a lab equipment product designed for the purification of high-quality total RNA from a wide variety of cell and tissue samples. It utilizes a silica-membrane-based technology to efficiently capture and purify RNA, enabling reliable and reproducible results.

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Lab products found in correlation

Lightcycler 480 dna sybr green 1 master chemistry, by Roche (2 mentions) Superscript 3 first strand synthesis supermix kit, by Thermo Fisher Scientific (2 mentions) Lightcycler 480, by Roche (2 mentions) Rneasy ffpe kit, by Qiagen (1 mentions) Quantitect probe rt pcr kit, by Qiagen (1 mentions)

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RNeasy Mini Kit (41418 citations) RNeasy kit (11240 citations) RNeasy Mini (299 citations) Mini Kits (17 citations) RNeasy kit Mini Kit (2 citations)

3 protocols using rneasy 96 mini kit

1

ISAV Detection in Aquaculture Samples

Cited 1 time
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Total RNA was extracted from organ samples or from gill swabs from all farms using the RNeasy 96 mini kit (Qiagen) following manufacturers protocols with minor modifications. Subsequently a duplex ISAV real-time RT-PCR (RT-qPCR) assay was used for detection of ISAV segment 8 and the endogenous control, elongation factor-1α (ELF) using the QuantiTect Probe RT-PCR kit (Qiagen) as previously described [12 (link)]. Primers and probe were used for ISAV [12 (link)] and ELF [19 (link)] as reported previously.
To investigate potential loss of virus and virus infected cells during preparation for histology 14 gills from farm II were re-tested post-formalin fixation and paraffin embedding. Total RNA was extracted from the paraffin embedded gill tissue blocks using the RNeasy FFPE kit (Qiagen) as recommended by the producer and the presence of ISAV was tested with the ISAV duplex RT-qPCR assay described above.
Aamelfot M., Christiansen D.H., Dale O.B., McBeath A., Benestad S.L, & Falk K. (2016). Localised Infection of Atlantic Salmon Epithelial Cells by HPR0 Infectious Salmon Anaemia Virus. PLoS ONE, 11(3), e0151723.
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2

Quantifying STAT3 Expression via RT-PCR

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RNA was isolated from 2 × 105 cells using the RNeasy 96 Mini Kit (Qiagen, Hiden, Germany) and converted to cDNA by the SuperScript III First-Strand Synthesis SuperMix kit (Invitrogen, Carlsbad, CA). Levels of STAT3 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts were determined by real-time polymerase chain reaction (RT-PCR) using LightCycler 480 DNA SYBR Green I Master chemistry on LightCycler 480 instrument (Roche, Basel, Switzerland). Results were expressed as the fold change versus the result for the corresponding GAPDH housekeeping gene mRNA values using the 2-ΔΔCt method.31 (link)
Yamada K., Huang Z.Q., Raska M., Reily C., Anderson J.C., Suzuki H., Ueda H., Moldoveanu Z., Kiryluk K., Suzuki Y., Wyatt R.J., Tomino Y., Gharavi A.G., Weinmann A., Julian B.A., Willey C.D, & Novak J. (2017). Inhibition of STAT3 Signaling Reduces IgA1 Autoantigen Production in IgA Nephropathy. Kidney International Reports, 2(6), 1194-1207.
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3

Quantitative RT-PCR for STAT1 Expression

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RNA was isolated from 2 × 105 cells using RNeasy 96 Mini Kit (Qiagen, Hilden, Germany), converted to complementary DNA by SuperScript III First-Strand Synthesis SuperMix kit (Invitrogen, Carlsbad, CA). Levels of STAT1 and GAPDH transcripts were determined by real-time quantitative polymerase chain reaction using LightCycler 480 DNA SYBR Green I Master chemistry on LightCycler 480 instrument (Roche, Basel, Switzerland), as reported before.20 (link) The primer sets for STAT1 and GAPDH genes are provided in the Supplementary Table S1. Results were expressed as the fold change versus the control groups after the results were normalized with corresponding GAPDH housekeeping gene mRNA values using the 2–ΔΔCT method.28 (link)
Yamada K., Huang Z.Q., Reily C., Green T.J., Suzuki H., Novak J, & Suzuki Y. (2023). LIF/JAK2/STAT1 Signaling Enhances Production of Galactose-Deficient IgA1 by IgA1-Producing Cell Lines Derived From Tonsils of Patients With IgA Nephropathy. Kidney International Reports, 9(2), 423-435.
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