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Fusion s western blot imager

Manufactured by Vilber
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Sourced in France

The Fusion S Western Blot Imager is a versatile laboratory instrument designed for the detection and analysis of proteins in Western blot applications. The device utilizes a high-resolution camera and advanced imaging technologies to capture and analyze chemiluminescent or fluorescent signals generated during the Western blot process.

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Lab products found in correlation

Hrp coupled secondary antibody, by Jackson ImmunoResearch (2 mentions) Pierce power blotter, by Thermo Fisher Scientific (2 mentions)

2 protocols using fusion s western blot imager

1

Western Blot Analysis of Protein Markers

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Cells on a thermo-shaker were lysed for 10 min at 95 °C in Laemmli buffer. Samples were size separated via SDS-PAGE. Proteins were transferred onto nitrocellulose membranes with Pierce™ Power blotter (Thermo Fisher Scientific, Waltham, MA, USA). The following antibody dilutions were used: Syn5 (1:1000), GS27 (1:1000), GS28 (1:1000), and GFP (1:5000). Corresponding HRP-coupled secondary antibodies (1:5000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) were used. Protein bands were visualized with SuperSignal (Thermo Scientific), and analyzed on a Fusion S western blot imager (Vilber Lourmat, Marne-la-Vallée, France). Densitometry analysis of the gels was carried out using ImageJ software from the NIH.
Forrester A., Rathjen S.J., Garcia-Castillo M.D., Bachert C., Couhert A., Tepshi L., Pichard S., Martinez J., Munier M., Sierocki R., Renard H.F., Valades-Cruz C.A., Dingli F., Loew D., Lamaze C., Cintrat J.C., Linstedt A.D., Gillet D., Barbier J, & Johannes L. (2020). Functional Dissection of the Retrograde Shiga Toxin Trafficking Inhibitor Retro-2. Nature chemical biology, 16(3), 327-336.
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2

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells on a thermo-shaker were lysed for 10 min at 95 °C in Laemmli buffer. Samples were size separated via SDS-PAGE. Proteins were transferred onto nitrocellulose membranes with Pierce™ Power blotter (Thermo Fisher Scientific, Waltham, MA, USA). The following antibody dilutions were used: Syn5 (1:1000), GS27 (1:1000), GS28 (1:1000), and GFP (1:5000). Corresponding HRP-coupled secondary antibodies (1:5000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) were used. Protein bands were visualized with SuperSignal (Thermo Scientific), and analyzed on a Fusion S western blot imager (Vilber Lourmat, Marne-la-Vallée, France). Densitometry analysis of the gels was carried out using ImageJ software from the NIH.
Forrester A., Rathjen S.J., Garcia-Castillo M.D., Bachert C., Couhert A., Tepshi L., Pichard S., Martinez J., Munier M., Sierocki R., Renard H.F., Valades-Cruz C.A., Dingli F., Loew D., Lamaze C., Cintrat J.C., Linstedt A.D., Gillet D., Barbier J, & Johannes L. (2020). Functional Dissection of the Retrograde Shiga Toxin Trafficking Inhibitor Retro-2. Nature chemical biology, 16(3), 327-336.
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