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Cd16 fitc 3g8

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The CD16 FITC (3G8) is a fluorescently labeled antibody that targets the CD16 antigen. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Cellsdirect 2x reaction mix, by Thermo Fisher Scientific (2 mentions) Cd3 alexa 700 sp34 2, by BD (2 mentions) Cd14 qdot605 tuk4, by Thermo Fisher Scientific (2 mentions) Hla dr pe cy7 g46 6, by BD (2 mentions) Cd45ra ecd 3p, by Beckman Coulter (2 mentions) Cd27 apc efluor780 o323, by Thermo Fisher Scientific (2 mentions) Cd8 pe sk1, by BD (2 mentions) Amine aqua amcyan, by Thermo Fisher Scientific (2 mentions) Rnaqueous micro lysis buffer, by Thermo Fisher Scientific (2 mentions) Cd4 qdot655 s3, by Thermo Fisher Scientific (2 mentions) Facsaria, by BD (2 mentions) Cd27 apc efluor780, by Thermo Fisher Scientific (1 mentions) Cd4 v450 clone rpa t4, by BD (1 mentions) Cd45ro pe cy7 uchl 1, by BD (1 mentions) Pan γδtcr pe clone immu510, by Beckman Coulter (1 mentions) Cd45 fitc 2d1, by BD (1 mentions) Cd3 efluor450 okt3, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Cd3 bv605 okt3, by BioLegend (1 mentions)

Close spelling variants of this product

CD16 FITC (clone 3G8) Anti-CD16 FITC (clone 3G8, CD16 (3G8, CD16-FITC

5 protocols using cd16 fitc 3g8

1

Multicolor Flow Cytometry Cell Sorting

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Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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2

Multicolor Flow Cytometry Cell Sorting

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Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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3

Flow Cytometry Antibody Panel

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Antibodies used for flow cytometry include: pan-γδTCR-PE (clone IMMU510; Beckman Coulter, Woerden, the Netherlands), pan-αβTCR-APC (clone IP26; eBioscience, Thermo Fisher Scientific), CD4-V450 (clone RPA-T4; BD Biosciences), CD8α-PerCP-Cy5.5 (RPA-T8; Biolegend), CD3-eFluor 450 (OKT-3; eBioscience), CD45-FITC (2D1; BD Biosciences), CD16-FITC (3G8; BD Biosciences), CD56-FITC (MY31; BD Biosciences), CD27-APC-eFluor780 (O323; eBioscience), CD45RO-PE-Cy7 (UCHL-1; BD Biosciences). All samples were analyzed on a BD LSRFortessa using FACSdiva software (BD Biosciences).
Straetemans T., Kierkels G.J., Doorn R., Jansen K., Heijhuurs S., dos Santos J.M., van Muyden A.D., Vie H., Clemenceau B., Raymakers R., de Witte M., Sebestyén Z, & Kuball J. (2018). GMP-Grade Manufacturing of T Cells Engineered to Express a Defined γδTCR. Frontiers in Immunology, 9, 1062.
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4

Comprehensive Immune Profiling by Flow Cytometry

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We used flow cytometry with hierarchical gating strategies adapted from established guidelines for analysis of human PBMCs and suitable for cryopreserved samples (18 (link), 19 (link)). Our panel offered survey phenotyping of T cell subsets, including Tregs, as well as B cells and NK cells (see Figure S1 in Supplementary Material). The following fluorochrome-conjugated monoclonal antibodies were used: CD3 BV605 (OKT3, Biolegend), CD4 Alexa 700 (RPA-T4, Biolegend), CD8α V500 (SK1, BD Biosciences), CCR6 PE-Cy7 (G034E3, Biolegend), CXCR3 PE (G025H7, Biolegend), CD127 APC (A019D5, Biolegend), CD25 BV421 (M-A251, Biolegend), CD19 V500 (HIB19, BD Biosciences), CD56 PE-Cy7 (HCD56, Biolegend), CD20 PE (2H7, BD Biosciences), CD14 V450 (MFP9, BD Biosciences), and CD16 FITC (3G8, BD Biosciences). We also used the commercially available IOTest® Beta Mark Kit (Beckman Coulter) for quantitative determination of the human TCR Vβ repertoire in CD4+ and CD8+ cells [nomenclature from Ref. (20 (link))].
Patas K., Willing A., Demiralay C., Engler J.B., Lupu A., Ramien C., Schäfer T., Gach C., Stumm L., Chan K., Vignali M., Arck P.C., Friese M.A., Pless O., Wiedemann K., Agorastos A, & Gold S.M. (2018). T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder. Frontiers in Immunology, 9, 291.
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5

Multicolor Flow Cytometry Analysis of TLR and Chemokine Receptor Expression

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PBMCs were stained with anti-HLA-DR-PerCP (L243), -LIN-1-FITC (CD3, CD14, CD16, CD19, CD20, CD56), -CD11c-AlexaFluor700 (B-ly6), -CD3-PerCP (SK7), -CD56-PE-Cy7 (NCAM16.2), -CD16-FITC (3G8) (BD Biosciences), -TLR1-PE (GD2.F4), -TLR2-APC (TL2.1) (ebioscience) and -CCR2-PerCP-Cy5.5 (TG5, Biolegend, San Diego, CA) monoclonal antibodies to detect mDC and NK cell TLR1, TLR2 and CCR2 expression, and analyzed by flow cytometry on a BD LSRII using FACS Diva software. TLR1, TLR2, and CCR2 expression was analyzed based on fluorescence minus one (FMO).
Judge C.J., Reyes-Aviles E., Conry S.J., Sieg S.S., Feng Z., Weinberg A, & Anthony D.D. (2015). HBD-3 induces NK Cell activation, IFN-γ secretion and mDC dependent cytolytic function. Cellular immunology, 297(2), 61-68.
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