Log In Sign up
The largest database of trusted experimental protocols

Anti flag hrp a8592

Manufactured by Abcam
Visit Supplier

Anti-FLAG-HRP (A8592) is a horseradish peroxidase (HRP) conjugated antibody that specifically recognizes the FLAG peptide tag. It can be used for the detection of FLAG-tagged proteins in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti ha hrp 2999s, by Merck Group (3 mentions) Anti mbd3 ab157464, by Leinco Technologies (3 mentions) Anti rabbit hrp r115, by Leinco Technologies (3 mentions) Anti hdac1 hrp, by Merck Group (2 mentions) Bolt 4 12 bis tris plus gel, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Anti-FLAG-HRP A8592 Anti-Flag

3 protocols using anti flag hrp a8592

1

Protein Interaction Analysis via Pulldown and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pulldown assays and western blot analyses used for the MTAN-HDAC-MBDGATAD2CC subcomplex (in Figure S3D) and PWWP2A interaction studies (in Figure 5) were performed essentially as described in Torrado et al. (2017) (link). Briefly, after FLAG-affinity purification (as described above), protein samples were separated via SDS-PAGE using Bolt™ 4%–12% Bis-Tris Plus gels (Thermo Fisher Scientific) run in MES buffer at 165 V for 45 min. The gel-separated proteins were blotted onto PVDF membranes and probed with antibodies at the following concentrations: from Cell Signaling Technology: anti-HA-HRP (2999S, 1:40,000) and anti-HDAC1-HRP (59581S, 1:80,000); from Sigma-Aldrich: anti-FLAG-HRP (A8592, 1:80,000); from Abcam: anti-MBD3 (ab157464, 1:2,500); from Leinco Technologies: anti-rabbit-HRP (R115, 1:10,000). Blots were imaged using enhanced chemiluminescence and X-ray films.
Low J.K., Silva A.P., Tabar M.S., Torrado M., Webb S.R., Parker B.L., Sana M., Smits C., Schmidberger J.W., Brillault L., Jackman M.J., Williams DC J.r., Blobel G.A., Hake S.B., Shepherd N.E., Landsberg M.J, & Mackay J.P. (2020). The Nucleosome Remodeling and Deacetylase Complex Has an Asymmetric, Dynamic, and Modular Architecture. Cell reports, 33(9), 108450.
+ Open protocol
+ Expand
2

Protein Interaction Analysis via Pulldown and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pulldown assays and western blot analyses used for the MTAN-HDAC-MBDGATAD2CC subcomplex (in Figure S3D) and PWWP2A interaction studies (in Figure 5) were performed essentially as described in Torrado et al. (2017) (link). Briefly, after FLAG-affinity purification (as described above), protein samples were separated via SDS-PAGE using Bolt™ 4%–12% Bis-Tris Plus gels (Thermo Fisher Scientific) run in MES buffer at 165 V for 45 min. The gel-separated proteins were blotted onto PVDF membranes and probed with antibodies at the following concentrations: from Cell Signaling Technology: anti-HA-HRP (2999S, 1:40,000) and anti-HDAC1-HRP (59581S, 1:80,000); from Sigma-Aldrich: anti-FLAG-HRP (A8592, 1:80,000); from Abcam: anti-MBD3 (ab157464, 1:2,500); from Leinco Technologies: anti-rabbit-HRP (R115, 1:10,000). Blots were imaged using enhanced chemiluminescence and X-ray films.
Low J.K., Silva A.P., Tabar M.S., Torrado M., Webb S.R., Parker B.L., Sana M., Smits C., Schmidberger J.W., Brillault L., Jackman M.J., Williams DC J.r., Blobel G.A., Hake S.B., Shepherd N.E., Landsberg M.J, & Mackay J.P. (2020). The Nucleosome Remodeling and Deacetylase Complex Has an Asymmetric, Dynamic, and Modular Architecture. Cell reports, 33(9), 108450.
+ Open protocol
+ Expand
3

Evaluating MTA N-HDAC-MBD Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pulldown assays and western blot analyses used for the MTA N -HDAC-MBD GATAD2CC subcomplex (in Figure S5) and PWWP2A interaction studies (in Figure 5) were performed essentially as described in (25) for proteins produced in HEK293 cells. Antibodies used for the western blots were from Cell Signalling Technology: anti-HA-HRP (2999S, 1:40,000) and anti-HDAC1-HRP (59581S, 1:80,000); from Sigma-Aldrich: anti-FLAG-HRP (A8592, 1:80,000); from Abcam: anti-MBD3 (ab157464, 1:2,500); from Leinco Technologies: anti-rabbit-HRP (R115, 1:10,000).
Low J.K.K., Silva A.P., Tabar M.S., Torrado M., Webb S.R., Parker B.L., Sana M., Smits C., Schmidberger J., Brillault L., Jackman M.J., Williams D., Blobel G.A., Hake S.B., Shepherd N.E., Landsberg M.J., & Mackay J.P. (2020). The Nucleosome Remodeling and Deacetylase complex has an asymmetric, dynamic, and modular architecture.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!