The CCK-8 assay was performed to assess cell viability. In brief, cells were inoculated in 96-well plates at a density of 8×103 cells/well, with different concentrations (20, 40, 80 and 160 µM) of artemether (Chemical Abstracts Service no. 71963-77-4; Toronto Research Chemicals) and incubated at 37°C for 24, 48 and 72 h, control without artemether. Following incubation, 10 µl CCK-8 reagent was added into each well and cells were cultured for another 2 h at 37°C. The absorbance in each well was measured at a wavelength of 450 nm using a microplate reader (BioTek Instruments, Inc.).
Artemether
Manufactured by LGC
Sourced in United States
Artemether is a laboratory reagent used in the analysis and measurement of various compounds. It is a derivative of the antimalarial drug artemisinin and is commonly employed in analytical procedures. The core function of Artemether is to serve as an analytical tool for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
Artemisinin, by Cayman Chemical (3 mentions)
Artesunate, by LGC (3 mentions)
Dihydroartemisinin, by LGC (3 mentions)
Deoxyartemisinin, by LGC (3 mentions)
Prestoblue, by Thermo Fisher Scientific (3 mentions)
Renilla glo, by Promega (3 mentions)
Xtt reagent, by American Type Culture Collection (3 mentions)
Amodiaquine hcl hydrate, by MedKoo Biosciences (3 mentions)
Imanitib, by MedKoo Biosciences (2 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
4 protocols using artemether
1
Cell Viability Assay with Artemether
2
Antimalarial Compound Screening Protocol
3
Antimalarial Compound Screening Protocol
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Unless otherwise stated all reagents were from Sigma-Aldrich (St. Louis, MO). DCM was from ThermoFisher (Waltham, MA, USA); artemisinin was from Cayman Chemical (Ann Arbor, MI, USA); artemether, artesunate, and dihydroartemisinin were gifts from Prof. J. Plaizier-Vercammen (Brussels, Belgium); deoxyartemisinin was from Toronto Research Chemicals (North York, ON, Canada); amodiaquine HCl hydrate (Cat #: 562290) and imanitib (Cat # 100956) were from Medkoo Biosciences Inc. (Morrisville, NC, USA); EMEM (Cat # 30–2003) and XTT reagent (Cat # 30–1011k) were from ATCC; PrestoBlue was from Life Technologies (Cat #P50201); Renilla-Glo was from Promega (E2720).
4
Antimalarial Drug Combination Screening
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Unless otherwise stated all reagents were from Sigma-Aldrich (St. Louis, MO, USA). DCM was from ThermoFisher (Waltham, MA, USA); artemisinin was from Cayman Chemical (Ann Arbor, MI, USA); artemether, artesunate, and dihydroartemisinin were gifts from Prof. J. Plaizier-Vercammen (Brussels, Belgium); deoxyartemisinin was from Toronto Research Chemicals (North York, ON, Canada); amodiaquine HCl hydrate (Cat #: 562290) and imatinib (Cat # 100956) were from Medkoo Biosciences Inc. (Morrisville, NC, USA); EMEM (Cat # 30–2003) and XTT reagent (Cat # 30-1011k) were from ATCC; PrestoBlue was from Life Technologies (Cat #P50201); Renilla-Glo was from Promega (E2720).
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