EndoCAb IgG, EndoCAb IgM, and EndoCAb IgA were measured using direct ELISA kits procured from Hycult Biotech. LBP and LL-37 were measured using sandwich ELISA kits procured from Hycult Biotech (Pennsylvania, USA). sCD14 and FABP2 were measured using sandwich ELISA kits from R&D systems (Minneapolis, USA). CXCL16 was analyzed using a sandwich ELISA kit procured from Thermo Scientific (Frederick, MD, USA). Galectin-3 was measured using sandwich ELISA kit (Abcam, MA, USA). Total IgG, IgA, and IgM were measured using sandwich ELISA kits procured from Invitrogen (Carlsbad, CA, USA). The samples were diluted in appropriate buffers, which contains HeteroBlock (Omega Biologicals, Bozeman, MT, USA) to block non-specific antibodies which may interfere with the assay. All the analyses were performed blinded to clinical status with the exception of galectin-3 and total immunoglobulins IgG, IgA, and IgM.
Sandwich elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
The Sandwich ELISA kit is a laboratory equipment used to detect and quantify specific proteins or analytes in a sample. It utilizes a solid-phase enzyme immunoassay technique to measure the concentration of the target analyte. The kit includes all the necessary reagents and components for the assay, such as capture and detection antibodies, standards, and buffers.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Agilent Technologies (6 mentions)
Endocab igm, by Hycult Biotech (4 mentions)
Endocab igg, by Hycult Biotech (4 mentions)
Endocab iga, by Hycult Biotech (4 mentions)
Direct elisa kits, by Hycult Biotech (4 mentions)
Sandwich elisa kit, by R&D Systems (4 mentions)
Gen5 v 1, by Agilent Technologies (4 mentions)
El800 microplate reader, by Agilent Technologies (3 mentions)
Standard griess reagent, by Cayman Chemical (3 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Sandwich elisa kits, by Hycult Biotech (2 mentions)
Sandwich elisa kit, by Abcam (2 mentions)
Spectramax plus 384, by Molecular Devices (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Rosettesep, by STEMCELL (1 mentions)
Microplate manager 4, by Bio-Rad (1 mentions)
Rat elisa kit, by Thermo Fisher Scientific (1 mentions)
El800, by Agilent Technologies (1 mentions)
Victor3 multilabel plate reader, by PerkinElmer (1 mentions)
Maxisorp, by Thermo Fisher Scientific (1 mentions)
81 protocols using sandwich elisa kit
1
Biomarker Measurement Protocols in Clinical Research
2
Quantification of Immune Biomarkers
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sCD14 and LBP were measured using sandwich ELISA kits procured from R&D systems (Minneapolis, USA) and Hycult Biotech (Pennsylvania, USA), respectively. EndoCAb IgG, EndoCAb IgA, and EndoCAb IgM were measured using direct ELISA kits procured from Hycult Biotech. CXCL16 was analyzed using a sandwich ELISA kit procured from Thermo Scientific (Frederick, MD, USA). Lysozyme levels were measured using sandwich ELISA kit procured from MBL (Massachusetts, USA). Total IgG, IgA, and IgM were measured using sandwich ELISA kits procured from Invitrogen (Carlsbad, CA, USA). To block non-specific antibodies that may interfere with the assay, the samples were diluted in appropriate buffers, which contained 50 μg/ml of HeteroBlock (Omega Biologicals, Bozeman, MT, USA) and kept for 30 minutes before adding into the ELISA plate. All the analyses were performed blinded to case/control and clinical status. In order to maintain the test quality and reproducibility, an internal control was included in all the assays and the coefficient of variation (CV) of replicates was set at ≤ 10%.
3
Quantification of Amyloid-Beta Levels
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After behavioral testing, all the mice were anesthetized and perfused transcardially with ice-cold normal saline. Brains were quickly removed, dissected at the midsagittal plane, and placed on ice. Tissues were homogenized in tissue protein extraction reagent buffer (Biosource International, Inc, Camarillo, CA, USA). 4-benzenesulfonyl ñuoride hydrochloride (Sigma Chemical Co,) was added to tissue lysates to prevent the degradation of Aβ. The concentrations of Aβ1-40 and Aβ1-42 were measured using a sandwich ELISA kit (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. The optical densities in each well were measured using a plate reader at 450 nm. A standard curve for the ELISA test was measured to determine the concentration of antigen in a sample, while a standard curve using a solution of known concentration was prepared in advance.
4
IL-8 Production Measurement by ELISA
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IL-8 production was determined by enzyme-linked immunosorbent assay (ELISA) as described previously (34 (link)). Briefly, AGS cells were pretreated with 2 μM RAP (Merck Millipore, Billerica, MA), a RAGE antagonist, for 1 h and exposed to 100 nM CDT holotoxin. After incubation for 24 h, the IL-8 concentration was measured by using a sandwich ELISA kit (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.
5
Quantifying Alzheimer's Biomarkers in Brain
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Fresh frozen left hindbrains were thoroughly homogenized in PBS buffer with 0.5% Triton X-100 (Sigma) and 1% protease inhibitor cocktail set I (Calbiochem), then processed as previously described [16 (link)]. After determination of protein concentration using the Pierce BCA Protein Assay Kit (Thermo Scientific), concentrations of Aβ1–42 and Aβ1–40 in the soluble and insoluble brain protein fractions were analyzed with sandwich ELISA kit (Invitrogen), per manufacturer’s instructions. The optical density of each well was read at 450 nm using the same microplate reader (Spectra Max 384 plus, Molecular Devices).
6
PON-1 Lactonase Activity Assay
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PON-1 lactonase activity toward delta-valerolactone (Sigma-Aldrich, Burlington, MA, USA) was determined [23 (link),24 (link)] using a modification of the method described by Khersonsky and Tawfik [25 (link)]. According to the manufacturer’s instructions, SAA was measured in serum using a commercially available sandwich ELISA kit (Invitrogen, Carlsbad, CA, USA) and absorbance was read at 450 nm using a microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) as previously reported [18 (link)].
7
Brain and CSF Protein Analysis
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Sandwich-ELISA kit was purchased from Invitrogen, and the assays were performed on brain lysates and CSF following the manufacturer's directions and using the antibodies provided in the kit.
8
Serum Amyloid A Measurement
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In the morning, 5 ml of fasting peripheral venous blood sample was taken from the patient and placed at room temperature to allow it to coagulate naturally for 10-20 minutes. Then, the coagulated blood sample was centrifuged for 30 minutes at 3000 rpm. After centrifugation, the upper serum was carefully separated, collected, and stored in a -80°C ultralow temperature refrigerator. SAA was measured from the stored serum using Invitrogen (Waltham, MA, USA) Sandwich ELISA kit according to the manufacturer's instructions.
9
Inflammatory Response of Macrophages to Brucella
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The inflammatory response in RAW264.7 macrophages and murine peritoneal cavity-derived macrophages infected with B. abortus or their respective controls was evaluated by measuring IL-6 and TNF-α cytokine levels in the supernatant at 1, 6, and 12 h. The concentrations of these pro-inflammatory cytokines were detected using a sandwich ELISA kit following the manufacturer’s specifications (Invitrogen). The absorbance was read on a Tecan nano-pro ELISA reader at 490 nm. The cytokine concentrations (pg/ml) were obtained by developing standard curves using recombinant IL-6 and TNF-α. All experiments were performed in triplicate.
10
Quantifying BMP2 in SKOV3 and OVSAHO Cells
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SKOV3 and OVSAHO cells were cultured to confluence in a complete medium. After washing with PBS, cells were incubated in serum-free medium with or without 500 μM CBDCA for an additional 24 h. After the medium had been passed through a 0.45 μm syringe filter, BMP2 was quantified by a sandwich ELISA kit from Invitrogen, according to the manufacturer’s instruction.
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