Anti jak

The recombinant human proteins VEGF and IL-6 were purchased from Pepro Tech (Suzhou, China). Anti-STAT3 (1:1000), anti-JAK (1:1000), anti-phospho-STAT3 (Y705) (1:1000), and anti-phospho-JAK (Y1007/1008) (1:1000) antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). The anti-CCDC7 (1:1000) antibody was purchased from Abcam (Cambridge, UK). Mouse anti-PCNA (1:1000) was obtained from BOSTER (China). The secondary antibody (goat anti-rabbit IgG) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). TransIT^®-2020 Transfection Reagent was purchased from Mirus (USA). The total RNA kit was purchased from Tian Gen (China), and the first-strand cDNA synthesis kit and SYBR Premix Ex Taq were purchased from TaKaRa (Shiga, Japan). The ELISA kits for IL-6 and VEGF were from DAKEWE (China). Tumor microarrays were purchased from Alenabio (Shanxi, China). All other kits or reagents were purchased from the Beyotime Institute of Biotechnology (Shanghai, China). The recombinant plasmid CCDC7-shRNA was obtained from Genechem (Shanghai, China). The plasmid was prepared using an Endofree Plasmid Giga kit purchased from Qiagen (Chatsworth, CA, USA).