Rabbit anti e cadherin 24e10

Rabbit anti-ACAT2 (1:1000) (ab131215) and rabbit anti-P21 (1:1000) (ab109520) were purchased from Abcam. Rabbit anti-SETD7 antibody(1:1000) (24840-1-AP), rabbit anti-P16-INK4A(1:1000) (10883-1-AP), rabbit anti-P27 (1:1000)(25614-1-AP), rabbit anti-Cyclin B1 (1:1000)(55004-1-AP), rabbit anti-Cyclin E1(1:1000)(11554-1-AP), mouse anti-Cyclin D1(1:1000)(60186-1-Ig), rabbit anti-MCM2(1:1000)(10513-1-AP), rabbit anti-Cortactin (1:400)(11381-1-AP) and rabbit anti-SMA (1:1000)(14395-1-AP), mouse anti-Vimentin(1:4000)(60330-1-Ig) were purchased from Proteintech. Rabbit anti-Snail2 (1:1000) (121235) was purchased from Brickell Biotech, Inc. Antibodies against YAP1(1:1000) (A1002), TAZ (1:1000) (A23034), TEAD1(1:1000) (A5218) were purchased from AB clonal. Rabbit anti-vinculin (1:1000) (E1E9V), rabbit anti-flag antibody (1:1000) (2272S), rabbit anti-myc antibody (1:1000) (14793S), rabbit anti-p53 (7F5) (1:1000)(2527S), rabbit anti-E-Cadherin (24E10) (1:1000)(3195S), rabbit anti-N-Cadherin (D4R1H) XP®(1:1000)(13116S) and mouse anti-ubiquitin (1:1000) (#3936) were purchased from Cell Signalling Technology.
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).

HeLa cells stably transfected with the indicated plasmids were lysed using RIPA lysis buffer (50 mM Tris–HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% Triton X-100 supplemented with 1 × cOmplete protease inhibitor cocktail (Sigma-Aldrich). The cell lysates were electrophoresed via SDS–PAGE, transferred onto PVDF membranes (Amersham), blocked with 5% non-fat dried milk (BD Biosciences), and incubated with appropriate primary and HRP-conjugated secondary antibodies (Bio-Rad Laboratories). Immunoblots were visualized using an ECL detection system (Santa Cruz Biotechnology). The Western blots were imaged using ChemiDoc MP (Bio-Rad). The following primary antibodies were used: rabbit anti-Myc (A14; Santa Cruz, 1:1,000), rabbit anti-E-cadherin (24E10; Cell Signaling Technology, 1:1,000), anti-vimentin (D21H3; Cell Signaling, 1:1,000), anti-N-cadherin (#32; BD Biosciences, 1:1,000), anti-β-catenin (6B3; Cell Signaling, 1:1,000), anti-β-tubulin (9F3; Cell Signaling, 1:1,000), and anti-DSG1 (H-290; Santa Cruz, 1:1,000). The full-length blots are shown in Supplementary data, Fig. S13.