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Oc43 spike

Manufactured by Sino Biological
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The OC43 spike is a laboratory product that consists of the spike protein from the OC43 coronavirus. The spike protein is a key structural component of the virus and plays a crucial role in the virus's ability to bind to and enter host cells. This product is intended for use in research and development applications.

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Lab products found in correlation

Mers spike, by Sino Biological (3 mentions) Nl63 spike, by Sino Biological (3 mentions) 229e spike, by Sino Biological (3 mentions) Hku1 spike, by Sino Biological (3 mentions) Goat anti human igg alexa fluor 647, by Jackson ImmunoResearch (3 mentions) Ique screener plus, by Intellicyt (2 mentions) Anti human iga alexa fluor 647, by Jackson ImmunoResearch (2 mentions) Anti human igm alexa fluor 647, by Jackson ImmunoResearch (2 mentions)

Close spelling variants of this product

HCoV-OC43 Spike (OC HCoV-OC43 spike proteins

3 protocols using oc43 spike

1

Multiplex Serological Profiling of SARS-CoV Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptavidin-coated beads with different intensities of phycoerythrin (PE)-channel fluorescence (Spherotech SVFA-2558–6K and SVFB-2558–6K) were incubated with the following biotinylated antigens: 10 μg/mL MERS spike (Sino Biological 40069-V08B), NL63 spike (Sino Biological 40604-V08B), 229E spike (Sino Biological 40605-V08B), HKU1 spike (Sino Biological 40606-V08B), OC43 spike (Sino Biological 40607-V08B), SARS-CoV-2 spike, SARS-CoV-2 RBD, SARS-CoV-2 NTD, SARS-CoV-1 spike, SARS-CoV-1 RBD or 10 μg/mL CD4 (56 (link)) as a control. SARS-CoV-2 and SARS-CoV-1 antigens were produced in-house as described above. Excess streptavidin sites were blocked with 10 μg/mL of CD4 and the beads were washed and mixed. The beads were stained with 1:50, 1:250 or 1:1250 plasma for 30 minutes at room temperature, washed, and stained with 2.5 μg/mL goat anti-human IgG Alexa Fluor 647 (Jackson Immunoresearch 109-606-170), anti-human IgA Alexa Fluor 647 (Jackson Immunoresearch 109-606-011) or anti-human IgM Alexa Fluor 647 (Jackson Immunoresearch 109-606-129). The samples were read with the iQue Screener Plus (Intellicyt) high-throughput flow cytometer and FACS data were analysed with Flowjo. Data from titrations were analysed by calculating area under the curve (AUC) for the titration and subtracting the AUC of the negative control antigen.
Cho H., Gonzales-Wartz K.K., Huang D., Yuan M., Peterson M., Liang J., Beutler N., Torres J.L., Cong Y., Postnikova E., Bangaru S., Talana C.A., Shi W., Yang E.S., Zhang Y., Leung K., Wang L., Peng L., Skinner J., Li S., Wu N.C., Liu H., Dacon C., Moyer T., Cohen M., Zhao M., Lee F.E., Weinberg R.S., Douagi I., Gross R., Schmaljohn C., Pegu A., Mascola J.R., Holbrook M., Nemazee D., Rogers T.F., Ward A.B., Wilson I.A., Crompton P.D, & Tan J. (2021). Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern. Science Translational Medicine, 13(616), eabj5413.
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2

SARS-CoV-2 Serology Assay Optimization

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Streptavidin-coated beads with different intensities of PE-channel fluorescence (Spherotech SVFA-2558–6K and SVFB-2558–6K) were incubated with the following biotinylated antigens: 10 μg/mL MERS spike, NL63 spike, 229E spike, HKU1 spike, OC43 spike (all from Sino Biological), SARS-CoV-2 spike, SARS-CoV-2 RBD, SARS-CoV-2 NTD, SARS-CoV-1 spike, SARS-CoV-1 RBD or 10 μg/mL CD4 as a control. Excess streptavidin sites were blocked with 10 μg/mL of CD4 and the beads were washed and mixed. The beads were stained with 1/50, 1/250 or 1/1250 plasma for 30 min at room temperature, washed, and stained with 2.5 μg/mL goat anti-human IgG Alexa Fluor 647 (Jackson Immunoresearch 109-606-170). The samples were read with the iQue Screener Plus (Intellicyt) high-throughput flow cytometer and FACS data were analysed with Flowjo. Data from titrations were analysed by calculating area under the curve (AUC) for the titration and subtracting the AUC of the negative control antigen.
Cho H., Gonzales-Wartz K.K., Huang D., Yuan M., Peterson M., Liang J., Beutler N., Torres J.L., Cong Y., Postnikova E., Bangaru S., Talana C.A., Shi W., Yang E.S., Zhang Y., Leung K., Wang L., Peng L., Skinner J., Li S., Wu N.C., Liu H., Dacon C., Moyer T., Cohen M., Zhao M., Lee F.E., Weinberg R.S., Douagi I., Gross R., Schmaljohn C., Pegu A., Mascola J.R., Holbrook M., Nemazee D., Rogers T.F., Ward A.B., Wilson I.A., Crompton P.D, & Tan J. (2021). Ultrapotent bispecific antibodies neutralize emerging SARS-CoV-2 variants. bioRxiv.
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3

Multiplexed SARS-CoV Antigen Binding Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptavidin-coated beads with different intensities of phycoerythrin (PE)–channel fluorescence (Spherotech, SVFA-2558-6K and SVFB-2558-6K) were incubated with the following biotinylated antigens: 10 μg/ml of Middle East respiratory syndrome (MERS) spike (Sino Biological, 40069-V08B), NL63 spike (Sino Biological, 40604-V08B), 229E spike (Sino Biological, 40605-V08B), HKU1 spike (Sino Biological, 40606-V08B), OC43 spike (Sino Biological, 40607-V08B), SARS-CoV-2 spike, SARS-CoV-2 RBD, SARS-CoV-2 NTD, SARS-CoV-1 spike, and SARS-CoV-1 RBD or CD4 (10 μg/ml) (56 (link)) as a control. SARS-CoV-2 and SARS-CoV-1 antigens were produced in-house as described above. Excess streptavidin sites were blocked with CD4 (10 μg/ml), and the beads were washed and mixed. The beads were stained with 1:50, 1:250, or 1:1250 plasma for 30 min at room temperature, washed, and stained with 2.5 μg/ml of goat anti-human IgG Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-170), anti-human IgA Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-011), or anti-human IgM Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-129). The samples were read with the iQue Screener Plus (IntelliCyt) high-throughput flow cytometer, and FACS data were analyzed with FlowJo. Data from titrations were analyzed by calculating the area under the curve (AUC) for the titration and subtracting the AUC of the negative control antigen.
Cho H., Gonzales-Wartz K.K., Huang D., Yuan M., Peterson M., Liang J., Beutler N., Torres J.L., Cong Y., Postnikova E., Bangaru S., Talana C.A., Shi W., Yang E.S., Zhang Y., Leung K., Wang L., Peng L., Skinner J., Li S., Wu N.C., Liu H., Dacon C., Moyer T., Cohen M., Zhao M., Lee F.E., Weinberg R.S., Douagi I., Gross R., Schmaljohn C., Pegu A., Mascola J.R., Holbrook M., Nemazee D., Rogers T.F., Ward A.B., Wilson I.A., Crompton P.D, & Tan J. (2021). Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern. Science Translational Medicine, 13(616), eabj5413.
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