Mito-TEMPO and MitoSOX were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against CHOP, BiP, GRP94, β-actin, and HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against SOD2 and caspase-12 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Sirt3 and acetyl-K68-SOD2 were obtained from Abcam (Cambridge, MA, USA). T-SOD assay kit, SOD2 assay kit, MDA assay kit, ATP assay kit, and mitochondrial isolation kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China).
Antibodies against chop
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against CHOP are laboratory reagents used to detect and study the CHOP protein, also known as DDIT3 or GADD153. CHOP is a transcription factor involved in the cellular stress response and apoptosis. These antibodies can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to analyze the expression and localization of CHOP in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Bca kit, by Thermo Fisher Scientific (3 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Mitochondrial isolation kit, by Beyotime (1 mentions)
Mitosox, by Merck Group (1 mentions)
T sod assay kit, by Beyotime (1 mentions)
Sod2 assay kit, by Beyotime (1 mentions)
Mitotempo, by Merck Group (1 mentions)
Hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Mda assay kit, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Sybr premix ex taq, by Takara Bio (1 mentions)
Alp assay kit, by Abcam (1 mentions)
Von kossa kit, by Abcam (1 mentions)
Primescript rt master mix, by Takara Bio (1 mentions)
Rnaiso plus, by Takara Bio (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Aldosterone, by Merck Group (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Grp78, by Cell Signaling Technology (1 mentions)
4 protocols using antibodies against chop
1
Mitochondrial Oxidative Stress Assays
2
Characterizing Bone Cell Differentiation
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Antibodies against CHOP, Runx2, β‐actin, ALP, cleaved caspase‐3, and ALP were purchased from Cell Signaling Tech (Danvers, MA, USA). Antibody against CD68 was purchased from R&D system (Minneapolis, MN, USA). ALP assay kit and Von Kossa kit were purchased from Abcam (Cambridge, MA, USA). Calcium diagnostic kit was from Teco Diagnostic (Anaheim, CA, USA). Lentiviral shRNA particles and siRNA for CHOP were purchased from Santa Cruz Biotech, Inc (Dallas, TX, USA). Controls were the ‘scrambled’ shRNA expression construct or siRNA from Santa Cruz Biotech. RNAiso Plus, PrimeScript™ RT Master Mix, and SYBR_Premix Ex Taq™ were purchased from Takara Bio Inc (Kusatsu, Japan).
3
Antibody-based Protein Expression Analysis
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Aldosterone and anti-β-actin antibody were purchased from Sigma. Antibodies against CHOP, glucose-regulated protein 78 (GRP78) and Caspase-3 were purchased from Cell Signaling Technology. Anti-CYPB antibodies were obtained from Abcam. All other chemicals were of analytical grade.
4
Diabetic Islet Cell Protection
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Dulbecco's modified Earle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific (Grand Island, NY, USA). Streptozotocin (STZ) and thapsigargin (TG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against disulfide-bond A oxidoreductase-like protein (DsbA-L) and CTRP9 were bought from Santa Cruz Biotechnology (Santa Cruz, CA, USA); antibodies against CHOP, GRP-78, TNF-α, Caspase-3, Caspase-9, Caspase-12, and GAPDH were from Cell Signaling Technology (Beverly, MA, USA). The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) kits were purchased from Roche Diagnostics (Mannheim, Germany). Globular CTRP9 proteins were purchased from Aviscera Bioscience (Santa Clara, CA, USA). Lactate dehydrogenase (LDH) assay kit was purchased from the Institute of Jiancheng Bioengineering (Nanjing, Jiangsu, China). The rat insulin ELISA kit was brought from Shanghai Enzyme-linked Biotechnology (Shanghai, China). The BCA kit was purchased from Thermo Fisher (Waltham, MA, USA). The rabbit anti-goat, goat anti-rabbit, and goat anti-mouse secondary antibodies were purchased from Zhongshan Company (Beijing, China).
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