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Mx3005p qrt pcr system

Manufactured by Takara Bio
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The Mx3005P qRT-PCR system is a real-time PCR instrument designed for quantitative reverse transcription PCR (qRT-PCR) analysis. It features an optical detection system, temperature control, and software for data analysis and reporting.

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Lab products found in correlation

Primescript rt reagent kit, by Takara Bio (5 mentions) Sybr green qrt pcr master mix, by Takara Bio (5 mentions) Trizol reagent, by Takara Bio (4 mentions)

Close spelling variants of this product

QRT-PCR system QRT-PCR MX3005P

5 protocols using mx3005p qrt pcr system

1

mRNA Expression Analysis via qRT-PCR

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For the mRNA analyses, the total RNA was extracted using the Trizol Reagent (TaKaRa) according to the protocol provided by the manufacturer. The total RNA was reversely transcribed using the PrimeScript RT reagent Kit (TaKaRa). The qRT-PCR for the analysis of mRNA expression was performed on a Stratagene Mx3005P qRT-PCR system using the SYBR Green qRT-PCR master mix (TaKaRa) and GAPDH for normalisation. The primers used for the amplification of the indicated genes are listed in Table S1. All of the samples were normalised to the internal controls, and the fold changes were calculated through relative quantification (2−ΔΔCt).
Gao F., Zhang Y., Wang S., Liu Y., Zheng L., Yang J., Huang W., Ye Y., Luo W, & Xiao D. (2014). Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer. Scientific Reports, 4, 3963.
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2

Quantitative RT-PCR analysis of mRNA

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For the mRNA analyses, the total RNA was extracted using the Trizol Reagent (Invitroen) according to the protocol provided by the manufacturer. The total RNA was reversely transcribed using the PrimeScript RT reagent Kit (TaKaRa). The qRT-PCR for the analysis of mRNA expression was performed on a Stratagene Mx3005P qRT-PCR system using the SYBR Green qRT-PCR master mix (TaKaRa) and GAPDH for normalisation. The primers used for the amplification of the indicated genes are listed in Table S1. All of the samples were normalised to the internal controls, and the fold changes were calculated through relative quantification (2−ΔΔCt).
Gao F., Huang W., Zhang Y., Tang S., Zheng L., Ma F., Wang Y., Tang H, & Li X. (2015). Hes1 promotes cell proliferation and migration by activating Bmi-1 and PTEN/Akt/GSK3β pathway in human colon cancer. Oncotarget, 6(36), 38667-38680.
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3

Quantifying Gene Expression in NPC Cells

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Total RNA was extracted from NPC cells using Trizol Reagent (TaKaRa, Dalian, China) according to the manufacturer’s instruction. Then mRNA was reversely transcribed to cDNA using the PrimeScript RT reagent Kit (TaKaRa). To evaluate the mRNA levels of a number of genes, qRT-PCR was performed on a Stratagene Mx3005P qRT-PCR System using SYBR Green qRT-PCR master mix (TaKaRa). GAPDH was used as the internal control. The primers used in qRT-PCR assay were listed in Supplementary Table 5. All samples were normalized to internal controls and fold changes were calculated based on relative quantification (2-∆∆Ct).
Li X., Zhao Z., Zhang X., Yang S., Lin X., Yang X., Lin X., Shi J., Wang S., Zhao W., Li J., Gao F., Liu M., Ma N., Luo W., Yao K., Sun Y., Xiao S., Xiao D, & Jia J. (2017). Klf4 reduces stemness phenotype, triggers mesenchymal-epithelial transition (MET)-like molecular changes, and prevents tumor progression in nasopharygeal carcinoma. Oncotarget, 8(55), 93924-93941.
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4

Quantifying Gene Expression in NPC Cells

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Total RNA was extracted from NPC cells using Trizol Reagent (TaKaRa, Dalian, China) according to the manufacturer's instruction. Then mRNA was reversely transcribed to cDNA using the PrimeScript RT reagent Kit (TaKaRa). To evaluate the mRNA levels of a number of genes, qRT-PCR was performed on a Stratagene Mx3005P qRT-PCR System using SYBR Green qRT-PCR master mix (TaKaRa). GAPDH was used as the internal control. The primers used in qRT-PCR assay were listed in Supplementary Table S5. All samples were normalized to internal controls and fold changes were calculated based on relative quantification (2−ΔΔCt).
Wang S.C., Lin X.L., Wang H.Y., Qin Y.J., Chen L., Li J., Jia J.S., Shen H.F., Yang S., Xie R.Y., Wei F., Gao F., Rong X.X., Yang J., Zhao W.T., Zhang T.T., Shi J.W., Yao K.T., Luo W.R., Sun Y, & Xiao D. (2015). Hes1 triggers epithelial-mesenchymal transition (EMT)-like cellular marker alterations and promotes invasion and metastasis of nasopharyngeal carcinoma by activating the PTEN/AKT pathway. Oncotarget, 6(34), 36713-36730.
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5

Colorectal Cancer Cell RNA Analysis

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Total RNA was extracted from the colorectal cancer cells using the Trizol reagent (TaKaRa) and reversely transcribed into cDNA using the PrimeScript RT reagent Kit (TaKaRa), according to the manufacturer's instructions. We analyzed mRNA expression with qRT-PCR on a Stratagene Mx3005P qRT-PCR system using the SYBR Green qRT-PCR master mix (TaKaRa) and GAPDH for normalization. All samples were normalized to the internal controls and the fold changes were calculated through relative quantification (2 -ΔΔCt ). Three independent experiments were carried out.
Wei F., Zhang T., Yang Z., Wei J.C., Shen H.F., Xiao D., Wang Q., Yang P., Chen H.C., Hu H., Chen Z.P., Huang Q., Li W.L, & Cao J. (2018). Gambogic Acid Efficiently Kills Stem-Like Colorectal Cancer Cells by Upregulating ZFP36 Expression. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 46(2).
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