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4 protocols using anti mouse ck 14

1

Protein Expression Analysis Protocol

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To determine the expression of target proteins, the following antibodies were used. Anti-CD163 (Abcam, Cambridge, MA, USA, ab182422), anti-CD80 (ThermoFisher Sci., MA5–15512), anti-iNOS (ThermoFisher Sci., PA1–036); anti-Stat1 (Cell Signaling, 9172 T), anti-p-Stat1 (Tyr701, Cell Signaling, 7649 T), anti-STAT3 (Cell Signaling, 9139 T), anti-p-Stat3 (Ser727, ThermoFisher Sci., 44–384G), anti-IL-12 (R&D, AF309-SP), anti-IL-10 (R&D, AF217-SP), anti-p-Smad3 (Novus Bio, Centennial, CO, USA, nbp1–77836), anti-PD-L1 (Abcam, ab205921, for western blot), anti-PD-L1 polyclonal antibody (Biorbyt, LLC, San Francisco, CA, USA, orb74809, for IHC), anti-β-Actin (Sigma-Aldrich, A1978); anti-mouse CK 8/18 (DSHB, Iowa City, IA, USA, Troma-I); anti-mouse CK 14 (BioLegend, San Diego, CA, USA, 905301); and anti-mouse F4/80 (eBioscience, Waltham, MA, USA, BM8).
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2

Comprehensive Antibody Protocol for Cell Analysis

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The following antibodies were used. Anti-β-Galactosidase (Bioss, bs-4960R), anti-alkaline phosphatase (Novus Biologicals, NBP1-32948); anti-heterochromatin protein 1-γ (HP1-γ, phospho Ser 93, Bioss, bs-3221R); anti-p21 (Bioss, bs-10129R); anti-p27 (phospho Thr 187, Abcam, ab75908); anti-NOS-1, 2 (Thermo Fisher Scientific, PA1-033 and -036); anti-NOS-3 (Sigma-Aldrich, SAB-4300435); anti-ErbB2 (Thermo Fisher Scientific, PA5-16395); anti-pSMAD3 (Ser423/Ser425, Novous Biological, NBP1-77836); anti-CD24 (Novus Biologicals, NBP1-4639055); anti-CD44 (Bioss, bs-2507R); anti-S-Nitroso-Cysteine (Abcam, ab50185 or Alpha Diagnostics, NISC11-A); anti-Integrin α6 (BD Biosciences, 555734); anti-GM130 (Cell Signaling, 12480 S); anti-human CK 14 (ThermoFisher, MA511599); anti-human CK 18 (ThermoFisher, PA514263); anti-mouse CK 14 (BioLegend, 905301); anti-human CK 8/18 (DSHB, Troma-I); anti-Cleaved Caspase3 (Cell Signaling, #9664); anti-β-Actin (Sigma, A1978); anti-DYNLL1 (Abcam, ab51603); and anti-ADMA (EMD Millipore, 09-814).
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3

Immunohistochemical Characterization of Cellular Markers

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The following antibodies were used: anti-integrin α6 (BD Biosciences, Franklin Lakes, NJ, USA, Cat. # 555734); anti-GM130 (IF: Cell Signaling Technology, Danvers, MA, USA, Cat. # 12480S; IHC: Novus Biologicals, Littleton, CO, USA, Cat. # NBP1-89756); anti-human CK 14 (Thermo Fisher, Waltham, MA, USA, Cat. # MA5-11599); anti-human CK 18 (Thermo Fisher, Waltham, MA, USA, Cat. # PA514263); anti-mouse CK 14 (BioLegend, San Diego, CA, USA, Cat. # 905301); anti-mouse CK 8/18 (DSHB, Iowa City, IA, USA, Cat. # Troma-I); anti-alpha smooth muscle actin (Abcam, Cambridge, UK, Cat. # Ab7817); anti-E-cadherin (BD Biosciences, Franklin Lakes, NJ, USA, Cat. # 610182); anti-vimentin (Life Technologies, Carlsbad, CA, USA, Cat. # PA5-86264); anti-PAR3 (Millipore, Burlington, MA, USA, Cat. # 07-330); anti-laminin alpha3 (Novus Biologicals, Littleton, CO, USA, Cat. # CL3112); and phospho-SMAD3 antibody (pS423/pS425, Novus Biologicals, Littleton, CO, USA, Cat. # NBP1-77836SS).
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4

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the expression of target proteins, the following antibodies were used. Anti-CD163 (Abcam, Cambridge, MA, USA, ab182422), anti-CD80 (ThermoFisher Sci., MA5–15512), anti-iNOS (ThermoFisher Sci., PA1–036); anti-Stat1 (Cell Signaling, 9172 T), anti-p-Stat1 (Tyr701, Cell Signaling, 7649 T), anti-STAT3 (Cell Signaling, 9139 T), anti-p-Stat3 (Ser727, ThermoFisher Sci., 44–384G), anti-IL-12 (R&D, AF309-SP), anti-IL-10 (R&D, AF217-SP), anti-p-Smad3 (Novus Bio, Centennial, CO, USA, nbp1–77836), anti-PD-L1 (Abcam, ab205921, for western blot), anti-PD-L1 polyclonal antibody (Biorbyt, LLC, San Francisco, CA, USA, orb74809, for IHC), anti-β-Actin (Sigma-Aldrich, A1978); anti-mouse CK 8/18 (DSHB, Iowa City, IA, USA, Troma-I); anti-mouse CK 14 (BioLegend, San Diego, CA, USA, 905301); and anti-mouse F4/80 (eBioscience, Waltham, MA, USA, BM8).
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