SCFA (acetate, propionate, and butyrate) content was determined by gas chromatography (Nelson 1020, Perkin-Elmer, St Quentin en Yvelines, France). The samples were extracted with water (wt g/vol), centrifuged at 17,000 × g for 10 min, and the supernatant collected. The proteins were precipitated using a phosphotungstic acid saturated solution. A volume of 0.1 mL of the supernatant was analyzed using a gas–liquid chromatograph (Autosystem XL; Perkin Elmer, Saint-Quentin-en-Yvelines, France). All samples were analyzed in duplicate. The data was collected and peaks integrated using Turbochromv6 software (Perkin Elmer, Courtaboeuf, France).
Turbochromv6
Manufactured by PerkinElmer
Sourced in France
Turbochromv6 software is a chromatography data system that provides data acquisition, processing, and reporting capabilities for analytical laboratories. The software supports a range of chromatographic techniques, including gas chromatography (GC) and liquid chromatography (LC).
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using turbochromv6
1
Quantification of Short-Chain Fatty Acids
2
Quantification of Short-Chain Fatty Acids
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The SCFA (acetate, propionate and butyrate) levels in the culture supernatants were determined by gas chromatography (Nelson 1020, Perkin Elmer, St Quentin-en-Yvelines, France). The overnight culture was centrifuged, and the supernatant was collected. The proteins were precipitated using a saturated phosphotungstic acid solution. A volume of 0.1 mL of the supernatant was analysed using a gas–liquid chromatograph (Autosystem XL; Perkin Elmer, Saint-Quentin-en-Yvelines, France). All the samples were analysed in duplicate. The data were collected, and the peaks were integrated using Turbochromv6 software (Perkin Elmer, Courtaboeuf, France).
3
Cecal Short-chain Fatty Acids Analysis
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Cecal content samples were extracted in water and proteins were precipitated by adding phosphotungstic acid. The supernatant fraction (0.1 μL) was analyzed on a gas–liquid chromatograph (Autosystem XL; Perkin Elmer, Saint-Quentin-en-Yvelines, France) using 2-ethylbutyrate as the internal standard. Data were collected and peaks integrated using Turbochrom v. 6 software (Perkin Elmer, Courtaboeuf, France) (Lan et al., 2007 (link)).
4
SCFA Analysis of Frozen Stool Samples
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SCFA analysis was carried out as described previously [32 (link)] using stool samples stored at −80 °C. Thawed samples were water-extracted and proteins were precipitated with phosphotungstic acid. SCFA analysis was performed using 0.1 μL of supernatant fraction and a gas-liquid chromatograph (CP7580; Agilent, Les Ulis, France) equipped with a split/splitless injector, a flame-ionization detector, and a capillary column (15 m × 0.53 mm, 0.5 μm) impregnated with SP 1000 (FSCAP Nukol; Supelco, Saint-Quentin-Fallavier, France). The carrier gas (H2) flow rate was 10 mL/min, and the inlet, column and detector temperatures were 200 °C, 100 °C and 240 °C, respectively. 2-Ethylbutyrate was used as internal standard. Samples were analyzed in duplicate. Data were collected and peaks integrated using the Turbochrom v 6 software (Perkin Elmer, Courtaboeuf, France).
5
Quantifying Cecal Short-Chain Fatty Acids
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Samples were water-extracted, and proteins were precipitated with phosphotungstic acid. A volume of 0.1 μl of the supernatant was analyzed for SCFAs on a gas–liquid chromatograph (Nelson 1020; Perkin-Elmer, St. Quentin en Yvelines, France) equipped with a split-splitless injector, a flame-ionization detector and a capillary column (15 m × 0.53 mm, 0.5 μm) impregnated with SP 1000 (FSCAP Nukol; Supelco, Saint-Quentin-Fallavier, France). The carrier gas (He) flow rate was 10 ml/min, and the inlet, column, and detector temperatures were 175, 100, and 280 °C, respectively. 2-Ethylbutyrate was used as the internal standard. Data were collected and peaks integrated using Turbochrom v6 software (Perkin Elmer, Courtaboeuf, France). Cecal SCFA concentrations are expressed as μmol/g of stool.
6
SCFA Quantification by Gas Chromatography
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SCFA (acetate, propionate, and butyrate) content was determined by gas chromatography (Nelson 1020, Perkin-Elmer, France). The samples were extracted with water (wt g/vol), centrifuged at 17,000 × g for 10 min, and then the supernatant collected. The proteins were precipitated using a phosphotungstic acid-saturated solution. A volume of 0.1 ml of the supernatant was analyzed using a gas–liquid chromatograph (Autosystem XL; PerkinElmer, France). All samples were analyzed in duplicate. The data were collected, and peaks were integrated using Turbochromv6 software (PerkinElmer).
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