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Ez link biotinylation kit nhs sulfo lc biotin

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The EZ-link biotinylation Kit (NHS-sulfo-LC-Biotin) is a laboratory reagent used for the reversible labeling of proteins with biotin. The kit contains NHS-sulfo-LC-biotin, which is a water-soluble, amine-reactive biotinylation reagent.

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2 protocols using ez link biotinylation kit nhs sulfo lc biotin

1

Biotinylation of Proteins for BLI Assay

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S-ecto, hACE2-Fc, and Bovine serum albumin (BSA) proteins were biotinylated using EZ-link biotinylation Kit (NHS-sulfo-LC-Biotin, ThermoFisher) with standard protocol. Briefly, 1 mL protein (2 mg/mL) in PBS was incubated with 20-fold molar excess of freshly prepared reagent for 30 min at RT. The reaction was stopped by 0.1 M glycine and then applied to Zeba spin desalting column (7k MWCO, 2 mL, ThermoFisher) in PBS. The eluent was used for BLI assay.
Fang Y., Sun P., Xie X., Du M., Du F., Ye J., Kalveram B.K., Plante J.A., Plante K.S., Li B., Bai X.C., Shi P.Y, & Chen Z.J. (2022). An antibody that neutralizes SARS-CoV-1 and SARS-CoV-2 by binding to a conserved spike epitope outside the receptor binding motif. Science Immunology.
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2

Biotinylation of Proteins for BLI Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
S-ecto, hACE2-Fc, and Bovine serum albumin (BSA) proteins were biotinylated using EZ-link biotinylation Kit (NHS-sulfo-LC-Biotin, ThermoFisher) with standard protocol. Briefly, 1 mL protein (2 mg/mL) in PBS was incubated with 20-fold molar excess of freshly prepared reagent for 30 min at RT. The reaction was stopped by 0.1 M glycine and then applied to Zeba spin desalting column (7k MWCO, 2 mL, ThermoFisher) in PBS. The eluent was used for BLI assay.
Fang Y., Sun P., Xie X., Du M., Du F., Ye J., Kalveram B.K., Plante J.A., Plante K.S., Li B., Bai X.C., Shi P.Y, & Chen Z.J. (2022). An antibody that neutralizes SARS-CoV-1 and SARS-CoV-2 by binding to a conserved spike epitope outside the receptor binding motif. Science Immunology.
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