Neochlorogenic acid

Reference substance: 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PS020247, purity: 98.0%), coniferyl ferulate (PS011045, purity: 95.0%), benzoylpaeoniflorin (PS000157, purity: 98.0%), methyl gallate (PS020247, purity: 98.0%), isoliquiritin apioside (PS020153, purity: 98.0%), isoliquiritin (PS012517, purity: 98.0%) were purchased from Chengdu Pusi Biotechnology Co., LTD; ferulic acid (110773–201313, purity: 99.6%), hesperidin (110721–201617, purity: 96.1%), liquiritin (111610–201908, purity: 95.0%), caffeic acid (110773–201614, purity: 99.0%), rutin (100080–201811, purity: 91.7%), chlorogenic acid (110753–201817, purity: 96.8%), gallic acid (110831–201204, purity: 89.9%), and narirutin (112007–201602, purity: 90.8%) were purchased from the China National Institute for Food and Drug Control; liquiritigenin (MUST-17022104, purity: 99.07%), glycyrrhizic acid (MUST-17022104, purity: 99.65%), albiflorin (MUST-18041601, purity: 99.14%), neochlorogenic acid (MUST-12113001, purity: 98.00%), and naringenin (MUST-16032406, purity: 99.18%) purchased from Chengdu MUST Biotechnology Co., Ltd. Methanol, formic acid, and acetonitrile (LC-MS grade) were purchased from Merck (Merck & Co., Inc.). Distilled water is supplied by Watsons (A. S. Watson TM Limited).

HPLC grade methanol and acetonitrile were obtained from Fisher Scientific (USA). HPLC grade formic acid was purchased from Dikma Co. (USA), and Wahaha purified water was obtained from Hangzhou Wahaha Group (China). Neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B (3,4-diCQA), isochlorogenic acid A (3,5-diCQA), isochlorogenic acid C (4,5-diCQA), bergapten, columbianetin acetate, isoimperatorin, osthol, and columbianadin were obtained from Chengdu Must Biotechnology Co., LTD (China), and columbianetin, xanthotoxin, psoralen, and chloramphenicol were obtained from Shanghai Nature Standard Biotechnology Co., LTD (China), and daidzein was obtained from Chengdu Purechem-Standard Biotechnology Co., LTD (China). The purity of the involved standards and reagents were over 98%, with their chemical structures exhibited in Figure 1.
The APR samples was obtained from Hubei Province (30°24N, 115°30E) picked in April 2019, and identified by Professor Lianjie Su from Heilongjiang University of Chinese Medicine, as the dry root of Angelica pubescens Maxim. f. biserrata Shan et Yuan and a voucher specimen were deposited at department of Chinese herbal medicine processing of Heilongjiang University of Chinese Medicine, Harbin, China (voucher numbers: APQH201904-7).

BSA was purchased from AMRESCO. Chlorogenic acid, cryptoChlorogenic acid and neoChlorogenic acid were all purchased from Chengdu Must Bio-Technology Company in China. Ibuprofen and Warfarin Sodium were purchased from Shanghai Yuanye Bio-Technology Company in China. Digoxin was purchased from Tokyo Chemical Industry Company in Japan. BSA solution was prepared to 1.0 × 10⁻⁴ M with water. PBS and Tris-HCl buffer were prepared to 5.0 × 10⁻² M, all the solution were kept at pH 7.4 and contain 0.1 M NaCl. The stock solution of 3-CQA, 4-CQA, 5-CQA, ibuprofen, warfarin sodium, and digoxin were directly dissolved in MeOH as stock solution (1.0 × 10⁻³ M).