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Anti pna fitc

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Anti-PNA-FITC is a fluorescently labeled antibody that binds to Peptide Nucleic Acid (PNA) molecules. It is designed for use in various laboratory applications that involve the detection and analysis of PNA-containing samples.

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Lab products found in correlation

Dm ire2 confocal microscope, by Leica (3 mentions) Cd4 apc, by Thermo Fisher Scientific (3 mentions) Igd pe, by Thermo Fisher Scientific (3 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Antifade reagent, by Thermo Fisher Scientific (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Fc block, by BD (1 mentions) Cyan flow cytometer, by Beckman Coulter (1 mentions) Anti cd3 fitc, by Thermo Fisher Scientific (1 mentions) Cellquest pro software, by BD (1 mentions) Sa alexa 647, by Thermo Fisher Scientific (1 mentions) Anti igd pe, by Southern Biotech (1 mentions)

Spelling variants of this product

FITC-PNA (67 citations)

4 protocols using anti pna fitc

1

GC Formation Analysis in Mice

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For GC formation analysis, the spleens from 1-year-old mice or SRBC-immunized mice were detached and frozen in OCT compound. Tissues were sectioned to 7 μm thickness, fixed in acetone at −20 °C, washed with PBS, and blocked with 0.1% BSA in PBS for 30 min at room temperature. Tissues were stained with anti-PNA-FITC (Sigma-Aldrich, St. Louis, MO, USA), IgD-PE, and CD4-APC (eBioscience) antibodies diluted in blocking solution overnight at 4 °C. The tissues were incubated with ProLong Gold anti-fade reagent (Life Technologies, Carlsbad, CA, USA) for 30 min at 4 °C, and images were obtained using a Leica DM IRE2 confocal microscope.
Kim D.H., Park H.J., Park H.S., Lee J.U., Ko C., Gye M.C, & Choi J.M. (2019). Estrogen receptor α in T cells suppresses follicular helper T cell responses and prevents autoimmunity. Experimental & Molecular Medicine, 51(4), 41.
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2

Immunohistochemical Analysis of Germinal Centers

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Mice aged 6 to 8 weeks old were immunized with sheep red blood cells (SRBC) and spleens were harvested 9 days later. Spleens from 1-year-old mice were also collected to analyze TFH cells and GC formation. The tissues were frozen in OCT reagent and sectioned into 7-µm slices. The frozen sections were stained with anti-PNA-FITC (Sigma-Aldrich, St. Louis, MO), IgD-PE, and CD4-APC (eBioscience) overnight at 4°C. After washing, Anti-Fade reagent (Invitrogen, Life Technologies, Carlsbad, CA) was added to the slides, which were visualized using a Leica DM IRE2 confocal microscope.
Park H.J., Kim D.H., Choi J.Y., Kim W.J., Kim J.Y., Senejani A.G., Hwang S.S., Kim L.K., Tobiasova Z., Lee G.R., Craft J., Bothwell A.L, & Choi J.M. (2014). PPARγ Negatively Regulates T Cell Activation to Prevent Follicular Helper T Cells and Germinal Center Formation. PLoS ONE, 9(6), e99127.
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3

Multi-color Flow Cytometry Protocol

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For cell surface staining the following mAbs were used (from BD Biosciences unless otherwise stated): Anti-CD3-FITC (145-2C11), anti-CD4-APC (RM4-5), anti-CD19-APC (1D3), anti-CD19-Pacific Blue (1D3, eBioscience), anti-CD43-Biotin (S7) detected with SA-Alexa-647 (Invitrogen), anti-CD43-FITC (S7), anti-CD44-FITC (IM7), anti-CD45-PercP (30F11), anti-CD69-PE (H1.2F3), anti-CD79 (polyclonal rabbit) detected with goat-anti-rabbit IgG-Alexa-649 (Molecular Probes), anti-CD79b-DyLight-488 (HM79), anti-CD79b-Dylight-649 (HM79), anti-CD80-PE (1610A1), anti-CD86-PE (GL-1), anti-B220-PerCP (RA3-6B2), anti-FcγRIIB-Dylight-488 (2.4G2), anti-Hamster IgG FITC (G70-204/G94.56), anti-IA/IE FITC (2G9), anti-IgD-PE (11-26, Southern Biotech), anti-IgM-Dylight-649 (b-7-6), anti-MHC-II-Dylight-488 (D3.137), and anti-PNA FITC (Sigma). Cells were incubated with Fc block (BD Biosciences) and stained in FACS buffer (PBS+1% BSA + 0.02% NaN3) to reduce non-specific staining. Flow cytometry was performed on cells using a CYAN flow cytometer (Beckman Coulter) or FACS Calibur flow cytometer (BD Biosciences) and analyzed using FlowJo (Treestar), CellQuestPro software (BD Biosciences) and PRISM (Graphpad Software).
Hardy I.R., Anceriz N., Rousseau F., Seefeldt M.B., Hatterer E., Irla M., Buatois V., Chatel L.E., Getahun A., Fletcher A., Cons L., Pontini G., Hertzberg N.A., Magistrelli G., Malinge P., Smith M.J., Reith W., Kosco-Vilbois M.H., Ferlin W.G, & Cambier J.C. (2014). Anti-CD79 Antibody Induces B cell Anergy That Protects Against Autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 192(4), 1641-1650.
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4

Immunohistochemical Analysis of GC Formation

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For GC formation analysis, the spleens from six- to eight-week-old sheep red blood cell (SRBC)-immunized mice were isolated 7 days after immunization and frozen in OCT compound. Tissues were sectioned to a 7-μm thickness, fixed in acetone at −20 °C, washed with PBS, and blocked with 0.1% BSA containing PBS for 30 min at room temperature. Tissues were stained with anti-PNA-FITC (Sigma-Aldrich, St. Louis, MO, USA), IgD-PE, and CD4-APC (eBioscience) antibodies diluted in blocking solution overnight at 4 °C. After three washes, tissues were incubated with ProLong Gold anti-fade reagent (Invitrogen, Life Technologies, Carlsbad, CA, USA) for 30 min at 4 °C and images were obtained using a Leica DM IRE2 confocal microscope.
Park H.J., Park H.S., Lee J.U., Bothwell A.L, & Choi J.M. (2016). Gender-specific differences in PPARγ regulation of follicular helper T cell responses with estrogen. Scientific Reports, 6, 28495.
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