Sem inspect f50

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SEM INSPECT F50 is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a field emission electron source, advanced optics, and a range of detectors to capture detailed information about the surface topography and composition of samples.

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Lab products found in correlation

Helios 600, by Thermo Fisher Scientific (1 mentions) Nova 200 nanolab, by Thermo Fisher Scientific (1 mentions) K550x sputter coater, by Quorum Technologies (1 mentions) Coulter ls230, by Beckman Coulter (1 mentions) Em cpd300 critical point dryer, by Leica camera (1 mentions)

10 protocols using sem inspect f50

Quantifying S. aureus Biofilm Formation

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S. aureus was grown overnight in TSB until stationary growth phase was reached. At this point, bacteria were adjusted to 10⁷ CFU/mL and added to a MW96 microplate and incubated at 37 °C for 16 h without shaking. After incubation, culture medium was discarded and biofilms were washed twice with PBS. In order to determine biofilm formation, Calcofluor White Stain (50 µL) was added to each well and incubated 1 min in the dark at room temperature. After incubation, the stain was removed and biofilms were washed twice with PBS. Samples were air-dried in the dark to be further visualized in an inverted fluorescence microscope (Olympus IX81).
To be analyzed by SEM, biofilms were grown on sterile glass slides incubated in a S. aureus planktonic suspension (10⁷ CFU/mL) at 37 °C for 16 h without shaking. Then, biofilms were washed twice with PBS (0.1 M) and fixed in 2.5% glutaraldehyde for 3 h. Samples were dehydrated through a series of ethanol solutions (30, 50, 70, 80, 90 and 100%; 15 min, twice). Finally, samples were air-dried at room temperature and coated with Pt to allow electronic observation. SEM images were acquired in the energy range of 10–15 keV in an SEM Inspect™ F50 (FEI Co., Hillsboro, OR, USA).

García-Salinas S., Elizondo-Castillo H., Arruebo M., Mendoza G, & Irusta S. (2018). Evaluation of the Antimicrobial Activity and Cytotoxicity of Different Components of Natural Origin Present in Essential Oils. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1399.

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Single-cell analysis of nanomaterial uptake

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Electron imaging was performed with a scanning electron microscope (SEM INSPECT F50.FEI company) and dual-beam (FIB/SEM. Helios 600.FEI company). SEM images were taken at 5 and 30 kV with a FEG column and a combined Ga-based 30 kV (10 pA) ion beam was used to cross-section single cells. The investigations were completed by energy-dispersive X-ray spectroscopy (EDX) for chemical analysis. For this, PC12 cells were seeded on glass coverslips (previously coated with poly-L-lysine) at a density of 5 × 10⁵ cells/ml. After cell adhesion, the growth media was removed and replaced with the reduced media containing the functionalized NPs (10 μg/ml) or media with corresponding NGF concentration. After 72 h of incubation, cells were washed with PBS, fixed, dehydrated, dried, and sputtered with 30 nm of gold for electron imaging.

Pinkernelle J., Raffa V., Calatayud M.P., Goya G.F., Riggio C, & Keilhoff G. (2015). Growth factor choice is critical for successful functionalization of nanoparticles. Frontiers in Neuroscience, 9, 305.

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Multimodal Imaging of MNP-Treated Cells

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SEM/focused ion beam (FIB) cross-sectioned cells were performed using scanning electron microscopy (SEM INSPECT F50; FEI, Hillsboro, OR) and dual-beam FIB/SEM (Nova 200 NanoLab; FEI). PC12 cells were grown on coverslips coated with PLL and treated with MNPs (10 μg mL⁻¹). After 24 h of incubation, the cells were washed with phosphate-buffered saline, fixed, and dehydrated. After drying, the samples were coated with 30 nm of gold. SEM images were taken at 5 and 30 kV with a field emission gun column, and a combined Ga-based 30 kV (10 pA) ion beam was used to cross-section single cells. These investigations were completed by energy dispersive x-ray analysis (EDX) for chemical analysis.

Raffa V., Falcone F., De Vincentiis S., Falconieri A., Calatayud M.P., Goya G.F, & Cuschieri A. (2018). Piconewton Mechanical Forces Promote Neurite Growth. Biophysical Journal, 115(10), 2026-2033.

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Bacterial Morphology Analysis by SEM

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To determine the effects of NP treatment, bacteria morphology was studied by SEM, as previously reported [18]. In brief, free cloxacillin and PLGA-cloxacillin NP (at respective MIC and 0.5 MIC) were added to S. aureus Newman and USA 300 strain cultures (final volume 1 ml, bacterial concentration: 5.10 6 cfu/ml) and incubated overnight under shaking (180 rpm, 37 • C).
Then, bacterial samples were washed twice (PBS 0.1 M) and fixed in glutaraldehyde (2.5%; 90 min). After fixation, samples were dehydrated in ethanol series (30-100%; twice for 15 min), air-dried and finally coated with Pt (15 nm) for electron microscopy imaging. SEM micrographs were recorded in a SEM Inspect F50 at 10-15 keV (FEI Co., LMA-INA, Spain).

Lacoma A., Usón L., Mendoza G., Sebastián V., Garcia-Garcia E., Muriel-Moreno B., Domínguez J., Arruebo M, & Prat C. (2020). Novel intracellular antibiotic delivery system against Staphylococcus aureus: cloxacillin-loaded poly(d,l-lactide-co-glycolide) acid nanoparticles. Nanomedicine (London, England), 15(12).

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Morphological Analysis of Samples

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The morphology of samples was determined using a Inspect F50 SEM (FEI Company, Eindhoven, The Netherlands), operated at an accelerating voltage from 1–30 kV. Samples (≈0.5 mg) were mounted on graphite tape to an aluminum stub. The powder was then sputter-coated with platinum (Emitech K550X, Qourum Technology, Lewes, UK).

Al-Hmoud L., Abu Fara D., Rashid I., Chowdhry B.Z, & Badwan A.A. (2020). Influence of Chitin Source and Polymorphism on Powder Compression and Compaction: Application in Drug Delivery. Molecules, 25(22), 5269.

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Nanotube Dispersion Analysis via SEM

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The dispersion of nanotubes in the nanocomposites was characterised using an Inspect F50 SEM (FEI, Oregon, USA) at an accelerating voltage of 10 kV. Samples were imaged from both the anterior and fractured side view.

Namasivayam M., Andersson M.R, & Shapter J. (2019). Role of Molecular Weight in Polymer Wrapping and Dispersion of MWNT in a PVDF Matrix. Polymers, 11(1), 162.

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Zein Extrudate Surface Morphology

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The surface morphology of zein extrudates prior and after testing in media was studied using SEM. Samples were mounted onto stubs using double-sided tape and were platinum coated by a Emitech K550 X sputter coater manufactured by Quorum Technologies (England). The imaging process was performed in a high vacuum environment. Imaging process was performed with a FEI Inspect F50 SEM (Netherlands), mounted with a tungsten filament with an acceleration voltage of 1-30 kV.

Bisharat L., Alkhatib H.S., Abdelhafez A., Barqawi A., Aljaberi A., Qi S, & Berardi A. (2020). Hot melt extruded zein for controlled delivery of diclofenac sodium: Effect of drug loading and medium composition. International journal of pharmaceutics, 585.

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Scanning Electron Microscopy Sample Preparation

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Samples were fixed onto aluminium stubs with double sided carbon tape and earthed with silver paint. SEM images were recorded on an FEI Inspect F50 SEM at an accelerating voltage of 5–10 keV, a working distance of approximately 10 mm, and processed with xT software.

Bird S.M., El-Zubir O., Rawlings A.E., Leggett G.J, & Staniland S.S. (2016). A novel design strategy for nanoparticles on nanopatterns: interferometric lithographic patterning of Mms6 biotemplated magnetic nanoparticles †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5tc03895b Click here for additional data file.. Journal of Materials Chemistry. C, Materials for Optical and Electronic Devices, 4(18), 3948-3955.

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Characterization of MNP Nanozymes

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Magnetic property of the MNP nanozymes was tested with a vibrating sample magnetometer (ADE 4HF VSM). The morphology of the polymeric MGs was measured by a scanning electron microscope (Inspect SEM F50, FEI Company). The size distribution of the MNPs and the wet MGs was evaluated using dynamic light scattering (DLS) with a Coulter LS230 instrument (Beckman-Coulter Co. Ltd.). The particle concentration for both MNPs and MGs was 0.1 mg mL⁻¹ during the testing.

Chen Z., Sellergren B, & Shen X. (2017). Synergistic Catalysis by “Polymeric Microzymes and Inorganic Nanozymes”: The 1+1>2 Effect for Intramolecular Cyclization of Peptides. Frontiers in Chemistry, 5, 60.

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Imaging Collagen Hydrogel Microstructure via SEM

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Scanning electron microscopy (SEM) images were acquired using an Inspect SEM F50 (FEI Company, US) in an energy range between 0 and 30 keV. 6 mg/ml collagen hydrogel was fabricated and polymerized in wells according the aforementioned protocol with no cellular component. Once the polymerization was completed, sample preparation procedure started by a drying stage using different ethanol concentrations in water. Then, samples were frozen separately in liquid nitrogen. Subsequently, samples were submitted to a critical point drying stage (Leica EM CPD300 Critical Point Dryer). Finally, the samples were coated with a carbon film before they were examined by SEM.
The mechanical properties of the collagen fibrous hydrogels used in this study were characterized in previous studies by our group.³⁶ The collagen hydrogel of 6 mg/ml presented a 0.69 μm ± 0.05 pore size, 90.53% ± 0.93 porosity and 254.05 Pa ± 29.06 storage shear modulus. Here, we imaged the micro‐architecture of the hydrogel by SEM. Finally, Image J specific tools were used to measure the collagen fibers thickness.

Alamán‐Díez P., García‐Gareta E., Arruebo M, & Pérez M.Á. (2022). A bone‐on‐a‐chip collagen hydrogel‐based model using pre‐differentiated adipose‐derived stem cells for personalized bone tissue engineering. Journal of Biomedical Materials Research. Part a, 111(1), 88-105.

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