An aliquot of the EX suspension (10 μL of vesicles in 90 μL of buffer) was collected after adding 10 μL of 10% glycine, which was then diluted with PBS to a final ratio of 1:200 before lysis with Mammalian Protein Extraction Reagent (Millipore, Billerica, MA, USA). The mean concentration (particles/mL) and diameter (nm) of EXs were calculated using NTA 3.1 nanoparticle tracking software and the Nanosight NS300 system with a G532-nm laser module (Malvern Instruments, Malvern, UK).
Mammalian protein extraction reagent
Manufactured by Merck Group
Sourced in United States
The Mammalian Protein Extraction Reagent is a laboratory product designed for the efficient extraction of proteins from mammalian cells and tissues. It is a ready-to-use solution that facilitates the isolation and purification of proteins for further analysis and experimentation.
Automatically generated - may contain errors
2 protocols using mammalian protein extraction reagent
1
EX Characterization by NTA Analysis
2
Exosome Diameter Measurement Protocol
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Exosome suspensions were collected after adding 10 μL of 10% glycine to 10 μL vesicles in 90 μL buffer, diluted with PBS to a final ratio of 1:200, and lysed with mammalian protein extraction reagent (Millipore, Billerica, MA, USA). Exosome diameters (nm) were calculated using a Nano Particle tracking analyzer (ZetaVIEW S/N 17-310, Particle Metrix, Germany).
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