Annexin 5 and pi kit

Manufactured by BD

The Annexin-V and PI kit is a laboratory reagent used for the detection and analysis of apoptosis, a form of programmed cell death. The kit contains Annexin-V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a fluorescent dye that binds to DNA. The binding of Annexin-V and PI to cells can be detected and quantified using flow cytometry or fluorescence microscopy, providing information about the stages of apoptosis in a cell population.

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Lab products found in correlation

Jc 1 reagent, by Beyotime (1 mentions) Cyan adp analyzer, by Beckman Coulter (1 mentions) Fc500 flow cytometry system, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Annexin V-FITC and PI detection kit Annexin V-FITC and PI apoptosis detection kit I Annexin V-FITC and PI apoptosis kit Annexin V-FITC and PI apoptosis detection kit Annexin V and PI apoptosis detection kit Annexin V-FITC and PI kit Annexin V-FITC and PI staining kit FITC-Annexin V and PE-PI apoptosis detection kit Annexin V and PI staining kit Dead Cell Apoptosis Kit with FITC-Annexin V and PI

2 protocols using annexin 5 and pi kit

TSLPR Expression and Apoptosis in Colon Cancer

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Colon cancer cells were stained with FITC-conjugated human anti-TSLPR antibody and with annexin-V and PI kit (BD Pharmingen). For the analysis of mitochondrial membrane potential, cancer cells were incubated with JC-1 reagent (Beyotime, China) according to the manufacturer's instruction. Cells were analyzed by CyAn™ ADP Analyzer (Beckman Coulter). All data was processed using Summit V5.2.0 program.

Yue W., Lin Y., Yang X., Li B., Liu J, & He R. (2016). Thymic stromal lymphopoietin (TSLP) inhibits human colon tumor growth by promoting apoptosis of tumor cells. Oncotarget, 7(13), 16840-16854.

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Apoptosis Analysis of miR-106a-5p Inhibitors

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Flow cytometry was utilized to determine the apoptotic rate of 0213SCG cells transfected with miR-106a-5p inhibitors and respective NC. Following transfection with miR-106a-5p inhibitors/NCs for 24 h, 0213SCG cells were harvested and stained using an Annexin V and PI kit (BD Bioscience) according to the manufacturers protocol. Subsequently, the mean fluorescence of the cells was measured and analyzed using the Beckman Coulter FC500 Flow Cytometry system with CXP software (Beckman Coulter, Inc.).

Xu H., Wang F, & Wang L. (2021). Suppression of miR-106a-5p expression inhibits tumorigenesis via increasing CELF-2 expression in spinal cord glioma. Oncology Letters, 22(2), 627.

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