Free amino acids were estimated by a high-performance liquid chromatographic (HPLC) system (Shimadzu Corporation, Kyoto, Japan) consisting of a system controller (SCL-6B), an auto-injector (SIL-6B), HPLC pumps (LC-6A), column oven (CTO-6A), fluorescence detector (RF-551), and a Supelcosil LC-18 column (5 mm packing, 150 × 4.6 mm). Briefly, 100 mg of sliced biological replicates was chosen randomly from each plot and dried to a constant weight for 16 h at 80 °C. The extraction was measured to determine free amino acids according to the method of Hansen [53 (link)]
Lc 18 column
Manufactured by Shimadzu
Sourced in Japan
The LC-18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The column features a stationary phase consisting of octadecylsilane (C18) bonded to silica particles, which provides effective separation of both polar and nonpolar analytes. The LC-18 column is suitable for a variety of analytical applications, including pharmaceutical, environmental, and food analysis.
Automatically generated - may contain errors
3 protocols using lc 18 column
1
Quantifying Free Amino Acids by HPLC
2
Analytical Characterization of Synthesized Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sample obtained from the optimal conversion
point was analyzed by high-performance liquid chromatography using
a Shimadzu LC-18 column at a temperature of 25 °C equipped with
a UV detector. A retention time of 5.5 min was observed for the product
in the injected sample (1 mg/mL), as shown inFigure S1 . The eluent was a mixture of acetonitrile and water
in a 4:1 (v/v) ratio, with a flow rate of 3.0 mL/min for 5 min. The
wavelength of 220 nm was chosen for the analysis, which showed the
highest absorption. The one-dimensional hydrogen (1H NMR)
and carbon (13C NMR) NMR spectra were recorded in a Bruker
spectrometer, model Advance DRX-300, belonging to the northeast center
for application and use of NMR, located at the Federal University
of Ceara (CENAUREMN-UFC). The experiment was carried out at a hydrogen
atom frequency of 300 MHz and a carbon atom frequency of 75 MHz. All
samples were dissolved in deuterated chloroform (CDCl3)
and analyzed in 5 mm NMR tubes.
point was analyzed by high-performance liquid chromatography using
a Shimadzu LC-18 column at a temperature of 25 °C equipped with
a UV detector. A retention time of 5.5 min was observed for the product
in the injected sample (1 mg/mL), as shown in
in a 4:1 (v/v) ratio, with a flow rate of 3.0 mL/min for 5 min. The
wavelength of 220 nm was chosen for the analysis, which showed the
highest absorption. The one-dimensional hydrogen (1H NMR)
and carbon (13C NMR) NMR spectra were recorded in a Bruker
spectrometer, model Advance DRX-300, belonging to the northeast center
for application and use of NMR, located at the Federal University
of Ceara (CENAUREMN-UFC). The experiment was carried out at a hydrogen
atom frequency of 300 MHz and a carbon atom frequency of 75 MHz. All
samples were dissolved in deuterated chloroform (CDCl3)
and analyzed in 5 mm NMR tubes.
3
Comparative Catechol Degradation Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The comparative analysis of catechol degradation
by Paracoccus sp. MKU1 and Paracoccus sp. MKU1 microcomposites was carried out by inoculating the bacteria
in MSM containing catechol (100 mg/L) and incubating at 37 °C.
Simply, 1 mL of culture supernatant was withdrawn at different time
intervals for centrifugation and the supernatant was filter-sterilized
through a 0.22 μm filter membrane. The residual concentration
of catechol was determined by high-performance liquid chromatography
using an LC-18 column (15 cm × 4.6 mm I.D., 5 μm, Shimadzu,
Japan) with a photoarray detector. The mobile phase for HPLC was 20
mM phosphoric acid and acetonitrile (75:25). The sample (20 μL)
was injected with 1.5 mL/min flow rate, and the compound was detected
at the wavelength of 270 nm.
by Paracoccus sp. MKU1 and Paracoccus sp. MKU1 microcomposites was carried out by inoculating the bacteria
in MSM containing catechol (100 mg/L) and incubating at 37 °C.
Simply, 1 mL of culture supernatant was withdrawn at different time
intervals for centrifugation and the supernatant was filter-sterilized
through a 0.22 μm filter membrane. The residual concentration
of catechol was determined by high-performance liquid chromatography
using an LC-18 column (15 cm × 4.6 mm I.D., 5 μm, Shimadzu,
Japan) with a photoarray detector. The mobile phase for HPLC was 20
mM phosphoric acid and acetonitrile (75:25). The sample (20 μL)
was injected with 1.5 mL/min flow rate, and the compound was detected
at the wavelength of 270 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!