Sn12c

SK-MEL-28 human melanoma cells were obtained from the Korean Cell Line Bank (Seoul, Korea). B16F10 mouse melanoma cells, A549 and NCI-H460 human lung cancer cells, SW480 and HCT116 human colon cancer cells, Caki-1 and SN12C human renal cancer cells, Ca Ski and C33A human cervical cancer cells, and MCF-7 and MDA-MB 231 human breast cancer cells were obtained from the American Type Culture Collection (Cryosite, Lane Cove NSW, Australia). A549, NCI-H460 and SW480 cells were grown in RPMI1640 (Gibco, Life Technologies, Grand Island, NY) with 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in 5% CO₂ humidified atmosphere. B16F10, Caki-1, SN12C, Ca Ski, C33A and MCF-7 cells were grown in DMEM (Gibco, Life Technologies, Grand Island, NY) with 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in 5% CO₂ humidified atmosphere. These cell lines were authenticated by monitoring of cell morphology, contamination inspection.

The human RCC cell lines used in this study, ACHN, A498, A704, Caki-1, Caki-2, SN12C, SNU-1272, and SNU4600, were purchased from the Korean Cell Line Bank (Seoul, Korea). ACHN, A498, A704, Caki-1, Caki-2, and SN12C cell lines were cultured in Dulbecco’s modified Eagle medium supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Waltham, MA, USA) and 10 µg/ml gentamicin (Gibco). SNU-1272 and SNU-4600 cell lines were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS and 10 µg/ml gentamicin.