Freshly thawed PBMC were used for surface and intracellular staining regarding flow cytometric analysis. The following monoclonal antibodies were used: Brilliant Violet (BV421)-conjugated anti-human PD-L1 (BD Biosciences), BV510-conjugated anti-human CD3 (BD Biosciences), fluorescein isothiocyanate (FITC)-conjugated anti-human CD4 (BD Biosciences), FITC-conjugated anti-human CD14 (BD Biosciences), (phycoerythrin (PE)-conjugated anti-human PD-1 (Beckmann-Coulter), PE-conjugated anti-human Gal-9 (Biolegend), Peridinin Chlorophyll Protein (PerCP)-conjugated anti-human CD56 (BD Biosciences), PerCP-conjugated anti-human Perforin (BD Biosciences), allophycocyanin (APC)-conjugated anti-human TIM-3 (R&D Systems), APC-conjugated anti-human CD56 (BD Biosciences), APC-conjugated anti-human FoxP3 (eBioscience), APC/H7-conjugated anti-human CD8 (BD Biosciences).
Fluorescein isothiocyanate fitc conjugated anti human cd4
Manufactured by BD
Sourced in United States
Fluorescein isothiocyanate (FITC)-conjugated anti-human CD4 is a fluorescent-labeled antibody that binds to the CD4 receptor on human cells. It is used in flow cytometry and other applications to detect and quantify CD4-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated anti human foxp3, by Thermo Fisher Scientific (2 mentions)
Brilliant violet bv421 conjugated anti human pd l1, by BD (2 mentions)
Bv510 conjugated anti human cd3, by BD (2 mentions)
Phycoerythrin pe conjugated anti human pd 1, by Beckman Coulter (2 mentions)
Apc h7 conjugated anti human cd8, by BD (2 mentions)
Fitc conjugated anti human cd14, by BD (1 mentions)
Apc conjugated anti human cd56, by BD (1 mentions)
Pe conjugated anti human gal 9, by BioLegend (1 mentions)
Peridinin chlorophyll protein percp conjugated anti human cd56, by BD (1 mentions)
Allophycocyanin apc conjugated anti human tim 3, by R&D Systems (1 mentions)
Fitc conjugated anti human cd107a, by BD (1 mentions)
Allophycocyanin apc conjugated anti human cd56, by BD (1 mentions)
2 protocols using fluorescein isothiocyanate fitc conjugated anti human cd4
1
Flow Cytometric Immunophenotyping of PBMCs
2
Comprehensive Immune Profiling of PBMC Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Thawed PBMC were used for surface and intracellular staining and analysis. The following monoclonal antibodies were used: Brilliant Violet (BV421)-conjugated anti-human PD-L1 (BD Biosciences, San Diego, CA, USA), BV510-conjugated anti-human CD3 (BD Biosciences, San Diego, CA, USA), fluorescein isothiocyanate (FITC)-conjugated anti-human CD4 (BD Biosciences, San Diego, CA, USA), FITC-conjugated anti-human CD107a (BD Biosciences, San Diego, CA, USA), phycoerythrin (PE)-conjugated anti-human PD-1 (Beckmann-Coulter, Miami, FL, USA), PE/Cy7-conjugated anti-human NKG2D (BD Biosciences, San Diego, CA, USA), allophycocyanin (APC)-conjugated anti-human CD56 (BD Biosciences, San Diego, CA, USA), APC-conjugated anti-human FoxP3 (eBioscience, San Diego, CA, USA), APC/H7-conjugated anti-human CD8 (BD Biosciences, San Diego, CA, USA).
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