Proteins in 2 × SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad), with the exception of histone H3 and GAPDH, which were separated on a 15% SDS polyacrylamide gel and transferred using a semi-dry blotting system (ATTO). After blocking with 5% skim milk (Nacalai Tesque), the membranes were incubated with the following primary antibodies: anti-Mcm2 antibody (D7G11; Cell Signaling Technology), anti-GAPDH antibody (14C10; Cell Signaling Technology), anti-histone H3 antibody (1G1, (41 (link))), anti-Cdc6 antibody (sc-9964; Santa Cruz Biotechnology), anti-Cdt1 (D10F11; Cell Signaling Technology), anti-Orc2 antibody (3G6; Santa Cruz Biotechnology), and anti-Cdc45 antibody (D7G6; Cell Signaling Technology). After incubation with peroxidase-conjugated secondary antibodies (GE Healthcare), the signals were detected using ImmunoStar LD (Fujifilm).
Anti mcm2 antibody d7g11
Manufactured by Cell Signaling Technology
The Anti-Mcm2 antibody (D7G11) is a reagent used for the detection of the Mcm2 protein in various applications. Mcm2 is a component of the MCM complex, which plays a crucial role in the initiation and regulation of DNA replication. This antibody can be used to study the expression and localization of Mcm2 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Skim milk, by Nacalai Tesque (2 mentions)
Anti gapdh antibody 14c10, by Cell Signaling Technology (2 mentions)
Anti cdc6 antibody sc 9964, by Santa Cruz Biotechnology (2 mentions)
Anti cdt1 d10f11, by Cell Signaling Technology (2 mentions)
Anti orc2 antibody 3g6, by Santa Cruz Biotechnology (2 mentions)
Anti cdc45 antibody d7g6, by Cell Signaling Technology (2 mentions)
Immobilon p pvdf membrane, by Merck Group (2 mentions)
Peroxidase conjugated secondary antibody, by GE Healthcare (2 mentions)
Anti histone h3 antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti mcm2 antibody d7g11
1
Western Blot Analysis of Cell Cycle Proteins
2
Western Blot Analysis of Cell Cycle Proteins
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Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad), with the exception of histone H3 and GAPDH, which were separated on a 15% SDS polyacrylamide gel and transferred using a semi-dry blotting system (ATTO). After blocking with 5% skim milk (Nacalai Tesque), the membranes were incubated with the following primary antibodies: anti-Mcm2 antibody (D7G11; Cell Signaling Technology), anti-GAPDH antibody (14C10; Cell Signaling Technology), anti-histone H3 antibody (1G1, ( 41)), anti-Cdc6 antibody (sc-9964; Santa Cruz Biotechnology), anti-Cdt1 (D10F11; Cell Signaling Technology), anti-Orc2 antibody (3G6; Santa Cruz Biotechnology), and anti-Cdc45 antibody (D7G6; Cell Signaling Technology). After incubation with peroxidase-conjugated secondary antibodies (GE Healthcare), the signals were detected using ImmunoStar LD (Fujifilm).
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