Anti rabbit hrp igg

Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) was provided by Gibco (Gai-thersburg, MD, USA). MTT, MPP⁺ and Als were obtained from Sigma-Aldrich (St. Louis, MO, USA). MitoTracker was purchased from Life Technologies (Carlsbad, CA, USA, Cat#1837173). The following antibodies were used: β-catenin (Abcam, Cambridge, UK, Cat#ab19449, RRID:AB_444927), PARP (Abcam, Cambridge, UK, Cat#ab32138, RRID:AB_777101), c-Myc (Abcam, Cambridge, UK, Cat# ab17356, RRID:AB_2148459), GAPDH (Cell Signaling, Beverly, MA, USA, Cat#3683, RRID:AB_1642205), GSK-3β (Cell Signaling, Beverly, MA, USA, Cat#12456, RRID:AB_2636978), p-GSK-3β (ser9; Cell Signaling, Beverly, MA, USA, Cat#9322P, RRID:AB_2115199), cleaved caspase-3 (Cell Signaling, Beverly, MA, USA, Cat#9669, RRID:AB_2069869), cleaved caspase-8 (Cell Signaling, Beverly, MA, USA, Cat#9496L, RRID:AB_2259431), β-actin (Biosynthesis biotechnology, Beijing, china), FITC or TRITC-conjugated secondary antibody (Biosynthesis biotechnology, Beijing, china, Cat#BSTEK021, Cat#BST12B15B31), Anti-mouse-HRP IgG or anti-rabbit -HRP IgG (Biosynthesis biotechnology, Beijing, china, Cat#RS0002, Cat#ZB2305).

DMEM and fetal bovine serum (FBS) were purchased from Gibco (Gaithersburg, MD). Propoxyphene (1 mg/ml, ampule of 1 ml) and MTT (purity≥98%) were obtained from Sigma-Aldrich (St. Louis, MO). Oxyhemoglobin (purity≥90%, Ruibio, 07109) was obtained from Bomei Biotechnology Co., Ltd. (Hefei, China). MitoTracker Red was purchased from Life Technologies (Massachusetts, USA). Antibodies to active-β-catenin, actin, and cleaved caspase-8 were purchased from Cell Signaling (Beverly, MA, USA). Antibodies to β-catenin, Bcl₂, Bax, and c-Myc were purchased from Abcam (Cambridge, UK). Anti-mouse-HRP IgG and anti-rabbit -HRP IgG were obtained from Biosynthesis Biotechnology (Beijing, China).