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Pc nc

Manufactured by Genechem
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Sourced in China

The Pc-NC is a compact, high-performance laboratory centrifuge designed for a wide range of applications in research and clinical settings. The core function of this equipment is to separate different components of a liquid sample, such as cells, organelles, or macromolecules, based on their density and size through the application of centrifugal force.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Si circ 0005615, by Genechem (1 mentions) Shrna nc, by Genechem (1 mentions)

2 protocols using pc nc

1

Modulating NOTCH1 Expression in Colorectal Cancer

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circ_0005615 small interfering RNA (si-circ_0005615; 5′-ATTTCGATCTTGAGCCAGA-3′) and the control (si-NC; 5′-CGTTATTACTTGAGCCAGA-3′), miR-665 mimic and negative control (miRNA NC), miR-665 inhibitor and negative control (inhibitor NC), NOTCH1 overexpression vector (pc-NOTCH1) and the control (pc-NC) were purchased from Genechem (Shanghai, China). The oligonucleotides or vectors were introduced into LOVO and SW480 cells at 60% confluency using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).
Wang X., Zheng D., Wang C, & Chen W. (2023). Knockdown of circ_0005615 enhances the radiosensitivity of colorectal cancer by regulating the miR-665/NOTCH1 axis. Open Medicine, 18(1), 20230678.
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2

Modulating MORC2 and NDRG1 in U251 cells

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A lentiviral expression vector (pLVX) containing two short hairpin (sh)RNAs targeting MORC2 (shRNA-MORC2-1 and sh-MORC2-2; 40 nM), shRNA negative control (shRNA-NC; 40 nM), the MORC2 plasmid (pc-MORC2; 4 µg), NDRG1 plasmid (pc-NDRG1; 4 µg) and empty vector plasmid (pc-NC; 4 µg) were designed and synthesized by Shanghai GeneChem Co. Ltd. U251 cells were plated into 6-well plates (1×106 cells per well). At 80% confluence, the transfection procedure was carried out using Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 48 h according to the manufacturer's protocol. The transfection efficiency was tested using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) or western blotting analysis after 48 h of transfection. The untransfected U251 cells were used as the control group.
Zhang J., Yang Y., Dong Y, & Liu C. (2021). Microrchidia family CW-type zinc finger 2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N-myc downstream regulated gene 1 promoter. International Journal of Molecular Medicine, 49(2), 16.
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